Linhart I, Weidenhoffer Z, Mráz J, Smejkal J, Mládková I
National Institute of Public Health, Centre of Industrial Hygiene and Occupational Diseases, Prague, Czech Republic.
Toxicol Lett. 1997 Feb 7;90(2-3):199-205. doi: 10.1016/s0378-4274(96)03849-0.
Male Wistar rats were dosed intraperitoneally with styrene (400 mg/kg). Urine samples were collected over phosphate buffer, pH 6.5 for 24 h. Excretion of mandelic (MA) and phenylglyoxylic acid (PGA) amounted to 1.66 +/- 0.62 and 5.21 +/- 2.44% of dose, respectively, as determined by ion-pair HPLC. After acidic hydrolysis, the amount of MA and PGA found in urine increased to 2.10 +/- 0.84 and 6.81 +/- 3.20% (mean +/- S.D.; n = 7), respectively. A similar increase was observed after alkaline hydrolysis of urine samples. Differences between hydrolysed and non-hydrolysed samples were significant in the paired t-test (P < 0.05). Further, urine samples were fractionated by HPLC. Fractions were subjected to acidic hydrolysis and analysed by HPLC and GC/MS. Both MA and PGA were detected in the fraction which did not contain any of these metabolites before hydrolytic treatment. Thus, MA and PGA, which are used as biomarkers of exposure to styrene, form hydrolysable conjugates in the rat. At least a minor part of the total urinary MA and PGA is bound in these conjugates.
将雄性Wistar大鼠腹腔注射苯乙烯（400毫克/千克）。在pH 6.5的磷酸盐缓冲液中收集尿液样本24小时。通过离子对高效液相色谱法测定，扁桃酸（MA）和苯乙醛酸（PGA）的排泄量分别占给药剂量的1.66±0.62%和5.21±2.44%。酸性水解后，尿液中MA和PGA的含量分别增加到2.10±0.84%和6.81±3.20%（平均值±标准差；n = 7）。对尿液样本进行碱性水解后也观察到类似的增加。在配对t检验中，水解样本和未水解样本之间的差异具有显著性（P < 0.05）。此外，通过高效液相色谱法对尿液样本进行分离。对分离出的组分进行酸性水解，然后通过高效液相色谱法和气相色谱/质谱联用仪进行分析。在水解处理前不含有任何这些代谢物的组分中检测到了MA和PGA。因此，作为苯乙烯暴露生物标志物的MA和PGA在大鼠体内形成了可水解的结合物。总尿MA和PGA中至少有一小部分与这些结合物结合。
