Keli E, Bouchoucha M, Devroede G, Carnot F, Ohrant T, Cugnenc P H
Hôpital Laennec, Départment de Gastroentérologie, Paris, France.
Dis Colon Rectum. 1997 Feb;40(2):222-8. doi: 10.1007/BF02054992.
Diversion-related colitis is an inflammatory process that affects the colon and/or rectum distal to a colostomy. Its mechanisms are unknown, and many hypotheses have been considered. The aim of the present study was to create an experimental model of diversion-related colitis in rats, so in the future it will be possible to test different hypotheses.
Three groups of ten male Wistar rats were used for the study. Two groups underwent a colostomy and were kept alive for 6 or 17 weeks. One group of rats was killed at the onset of the experiment. Specimens were taken in bypassed segments in the rats who had had a colostomy and in the sigmoid colon for the control group. Histologic analysis using standard coloration, histochemical techniques, and bacterial preparation was used to find histologic or changes of colonic histology or flora.
Exclusion was associated with vascular congestion, a decrease in length of glandular crypts (P < 0.01), and an erosion of surface epithelium; inflammation of the mucosa was absent in all control animals and present in all test animals. In contrast, the number of goblet cells was not changed by the procedure. There was also a significant change in distribution and intensity of sulfomucins and sialomucins and quantitative and qualitative changes of the colonic flora.
This experimental model of diversion colitis is characterized by histologic and bacteriologic modifications comparable with those reported in humans but with different histochemical changes.
改道相关性结肠炎是一种影响结肠造口术远端结肠和/或直肠的炎症过程。其发病机制尚不清楚，已有多种假说。本研究的目的是建立大鼠改道相关性结肠炎的实验模型，以便将来能够检验不同的假说。
三组，每组十只雄性Wistar大鼠用于本研究。两组大鼠接受结肠造口术，并存活6周或17周。一组大鼠在实验开始时处死。对接受结肠造口术的大鼠的旷置段以及对照组的乙状结肠取材。采用标准染色、组织化学技术和细菌制备进行组织学分析，以发现结肠组织学或菌群的组织学变化。
肠管旷置与血管充血、腺隐窝长度缩短（P<0.01）以及表面上皮糜烂有关；所有对照动物均无黏膜炎症，而所有实验动物均有黏膜炎症。相比之下，杯状细胞的数量未因该手术而改变。硫酸黏蛋白和唾液酸黏蛋白的分布和强度也有显著变化，结肠菌群也有定量和定性的变化。
这种改道性结肠炎实验模型的特点是组织学和细菌学改变与人类报道的相似，但组织化学变化不同。
