Watts D M, Phillips I, Callahan J D, Griebenow W, Hyams K C, Hayes C G
U.S. Naval Medical Research Institute Detachment, Lima, Peru.
Am J Trop Med Hyg. 1997 Feb;56(2):148-52. doi: 10.4269/ajtmh.1997.56.148.
Seroepidemiologic studies were conducted to determine the prevalence of Oropouche (ORO) viral antibody, risk factors, and the incidence of infection among residents of the Amazon region of Peru. Blood samples, as well as demographic, cultural, and medical history data, were collected from residents in a sector of the city of Iquitos and in an adjacent rural and three neotropical rain forest communities. Blood specimens were obtained approximately one year later from a cohort of the same study subjects who were negative for ORO antibody on the initial cross-sectional survey. Sera were tested for ORO IgG antibody by an enzyme-linked immunosorbent assay. Antibody prevalences were 35% for residents of the urban population, 24-46% for the forest communities, and 18% for the rural community. Antibody prevalence increased with age, and subjects who were seropositive were significantly (P = 0.001) older (mean = 33 years) than the seronegative subjects (mean = 15 years). Multivariate analysis revealed that only age, urban and forest residence, and occupation as a farmer or housekeeper remained significantly associated with seropositivity. Seroconversion data for the same populations one year later demonstrated evidence of ORO viral infection among 28% of the residents in the rural community and 2% or less in the forest and urban communities. Oropouche virus infection was significantly associated with older age (P = 0.04) in the rural community (P < 0.001). These data support prior evidence of ORO viral infection among residents of Iquitos and surrounding villages and suggest that transmission of this virus occurs continuously in the population of this area of the Amazon basin.
开展血清流行病学研究以确定秘鲁亚马逊地区居民中奥罗普切（ORO）病毒抗体的流行情况、危险因素及感染发生率。从伊基托斯市一个区以及相邻农村和三个新热带雨林社区的居民中采集血样以及人口统计学、文化和病史数据。大约一年后，从同一研究对象队列中获取血样，这些对象在初始横断面调查中ORO抗体呈阴性。通过酶联免疫吸附测定法检测血清中的ORO IgG抗体。城市居民的抗体流行率为35%，森林社区为24 - 46%，农村社区为18%。抗体流行率随年龄增长而升高，血清阳性者（平均年龄 = 33岁）比血清阴性者（平均年龄 = 15岁）显著年长（P = 0.001）。多变量分析显示，只有年龄、城市和森林居住情况以及农民或家庭主妇职业与血清阳性仍显著相关。一年后同一人群的血清转化数据表明，农村社区28%的居民有ORO病毒感染证据，森林和城市社区感染率为2%或更低。在农村社区，奥罗普切病毒感染与年龄较大显著相关（P = 0.04）（P < 0.001）。这些数据支持了伊基托斯及其周边村庄居民中存在ORO病毒感染的先前证据，并表明该病毒在亚马逊盆地这一地区的人群中持续传播。
