Baisley K J, Watts D M, Munstermann L E, Wilson M L
Department of Epidemiology and Public Health, Yale University School of Medicine, New Haven, Connecticut 06520-8034, USA.
Am J Trop Med Hyg. 1998 Nov;59(5):710-6. doi: 10.4269/ajtmh.1998.59.710.
A cross-sectional serosurvey of a rural community near Iquitos, Peru was conducted to determine Oropouche (ORO) virus antibody prevalence and risk factors for human infection. Venous blood samples, and demographic, social, and risk factor data were obtained from people age five years of age and older who lived in the village of Santa Clara on the Nanay River, a tributary of the Amazon River. Sera were tested for ORO viral antibody by an ELISA. The specificity of viral antibody reactivity was determined by a standard plaque-reduction neutralization test. Interview data were analyzed by univariate and multiple logistic regression to determine which variables were statistically associated with previous ORO viral infection, as indicated by the presence of IgG antibody. Final models were evaluated based on log-likelihood and Wald chi-square. Clustering of seropositive residents within houses was analyzed by the method of Walter. Among 1,227 persons sampled, 33.7% (n=414) were positive for ORO viral IgG antibody. Overall, antibody prevalence was similar for males (33.9%) and females (33.6%), and increased significantly with age for both sexes to include more than half of persons more than 25 years of age. The length of residence in the village was positively associated with serologic status; persons who had moved to the village within the past 15 years were less likely to be seropositive than life-long residents of the same age. Antibody prevalence among immigrants who had lived in Santa Clara more than 15 years was similar to that in life-long residents. The activity most predictive of previous ORO viral infection was travel to forest communities and travel to Iquitos. No evidence of spatial heterogeneity in ORO virus antibody distribution was observed. Results suggested that endemic transmission of ORO virus in this region has been ongoing during many decades, and that people are at considerable risk of infection.
在秘鲁伊基托斯附近的一个农村社区开展了一项横断面血清学调查，以确定奥罗波切（ORO）病毒抗体流行率及人类感染的风险因素。采集了居住在亚马逊河支流纳奈河上圣克拉拉村5岁及以上人群的静脉血样本、人口统计学、社会和风险因素数据。采用酶联免疫吸附测定法（ELISA）检测血清中的ORO病毒抗体。通过标准蚀斑减少中和试验确定病毒抗体反应性的特异性。采用单因素和多因素逻辑回归分析访谈数据，以确定哪些变量与既往ORO病毒感染在统计学上相关，既往感染通过IgG抗体的存在来表明。基于对数似然值和Wald卡方对最终模型进行评估。采用沃尔特方法分析房屋内血清阳性居民的聚集情况。在1227名抽样人员中，33.7%（n = 414）的ORO病毒IgG抗体呈阳性。总体而言，男性（33.9%）和女性（33.6%）的抗体流行率相似，且两性的抗体流行率均随年龄显著增加，25岁以上人群中超过一半呈阳性。在村里的居住时间与血清学状态呈正相关；在过去15年内搬到村里的人比同年龄的长期居民血清阳性的可能性更小。在圣克拉拉居住超过15年的移民中的抗体流行率与长期居民相似。最能预测既往ORO病毒感染的活动是前往森林社区和前往伊基托斯。未观察到ORO病毒抗体分布存在空间异质性的证据。结果表明，该地区ORO病毒的地方性传播已持续数十年，人们面临相当大的感染风险。
