Matsuda H, Wu J X, Tanaka T, Iinuma M, Kubo M
Faculty of Pharmaceutical Sciences, Kinki University, Osaka, Japan.
Biol Pharm Bull. 1997 Mar;20(3):243-8. doi: 10.1248/bpb.20.243.
The effects of 70% methanol extract (EA-ext) from Evodiae Fructus (EA) consisting of dried fruits of Evodia rutaecarpa var. bodinieri (Rutaceae) on nociceptive responses were investigated. Oral administration of 50 or 200 mg/kg EA-ext had the same antinociceptive effect on writhing responses as induced by acetic acid. Its major alkaloidal constituents, evodiamine and rutaecarpine also had the antinociceptive effect. EA-ext significantly decreased the frequency of licking behavior within a unit of time at the late phase without affecting that of the early phase in the formalin test. EA-ext also increased nociceptive threshold of the inflamed paw without increasing that in the non-inflamed paw in the Randall-Selitto test. Although EA-ext inhibited the rise of vascular permeability induced by acetic acid and the increase of paw edema induced by carrageenin, it was ineffective on nociceptive response in the hot plate test and on locomotor activity. These results suggest that EA possesses antinociceptive effects and its mode of action may be mediated by anti-inflammatory action, and that the antinociceptive constituents are only partially attributable to alkaloidal components mentioned above.
研究了由吴茱萸（芸香科，吴茱萸变种）干燥果实制成的吴茱萸70%甲醇提取物（EA-ext）对伤害性反应的影响。口服50或200mg/kg的EA-ext对醋酸诱导的扭体反应具有相同的抗伤害作用。其主要生物碱成分吴茱萸碱和吴茱萸次碱也具有抗伤害作用。在福尔马林试验中，EA-ext显著降低了后期单位时间内的舔舐行为频率，而不影响早期的频率。在Randall-Selitto试验中，EA-ext还提高了炎症爪的伤害性阈值，而未提高未发炎爪的阈值。虽然EA-ext抑制了醋酸诱导的血管通透性升高和角叉菜胶诱导的爪肿胀增加，但在热板试验中对伤害性反应和运动活动无效。这些结果表明，EA具有抗伤害作用，其作用方式可能通过抗炎作用介导，且抗伤害成分仅部分归因于上述生物碱成分。
