Greene A C, Patel B K, Sheehy A J
Microbiology Research Unit, Faculty of Applied Science, University of Canberra, Belconnen, Australian Capital Territory.
Int J Syst Bacteriol. 1997 Apr;47(2):505-9. doi: 10.1099/00207713-47-2-505.
A thermophilic anaerobic bacterium, designated strain BMAT (T = type strain), was isolated from the production water of Beatrice oil field in the North Sea (United Kingdom). The cells were straight to bent rods (1 to 5 by 0.3 to 0.5 microns) which stained gram negative. Strain BMAT obtained energy from the reduction of manganese (IV), iron(III), and nitrate in the presence of yeast extract, peptone, Casamino Acids, tryptone, hydrogen, malate, acetate, citrate, pyruvate, lactate, succinate, and valerate. The isolate grew optimally at 60 degrees C (temperature range for growth, 50 to 65 degrees C) and in the presence of 2% (wt/vol) NaCl (NaCl range for growth, 0 to 5% [wt/vol]). The DNA base composition was 34 mol% G + C. Phylogenetic analyses of the 16S rRNA gene indicated that strain BMAT is a member of the domain Bacteria. The closest known bacterium is the moderate thermophile Flexistipes sinusarabici (similarity value, 88%). Strain BMAT possesses phenotypic and phylogenetic traits that do not allow its classification as a member of any previously described genus; therefore, we propose that this isolate should be described as a member of a novel species of a new genus, Deferribacter thermophilus gen. nov., sp. nov.
从北海（英国）比阿特丽斯油田的采出水中分离出一株嗜热厌氧菌，命名为BMAT菌株（T = 模式菌株）。细胞为直杆状至弯杆状（1至5×0.3至0.5微米），革兰氏染色阴性。BMAT菌株在酵母提取物、蛋白胨、酪蛋白氨基酸、胰蛋白胨、氢气、苹果酸、乙酸、柠檬酸、丙酮酸、乳酸、琥珀酸和戊酸存在的情况下，通过还原锰（IV）、铁（III）和硝酸盐获取能量。该分离株在60℃时生长最佳（生长温度范围为50至65℃），在2%（重量/体积）NaCl存在的情况下生长（生长的NaCl范围为0至5%[重量/体积]）。DNA碱基组成为34摩尔%G + C。对16S rRNA基因的系统发育分析表明，BMAT菌株是细菌域的成员。已知最接近的细菌是嗜温菌Flexistipes sinusarabici（相似性值为88%）。BMAT菌株具有的表型和系统发育特征使其不能被归类为任何先前描述的属的成员；因此，我们建议将该分离株描述为一个新属嗜热脱铁杆菌属（Deferribacter thermophilus）的一个新物种的成员，即嗜热脱铁杆菌新属，新种。
