Zhang Y, Lewis K
Department of Biology, Massachusetts Institute of Technology, Cambridge 02139, USA.
FEMS Microbiol Lett. 1997 Apr 1;149(1):59-64. doi: 10.1111/j.1574-6968.1997.tb10308.x.
Two new antimicrobial peptides related to the gamma-thionine family have been isolated by acid extraction from the broad bean Vicia faba. The extract was separated by ion exchange chromatography, and a fraction showing antibacterial activity was further purified by reverse-phase HPLC. Material from a single HPLC peak was sequenced and revealed the presence of two peptides differing by one amino acid. The peptides were named fabatins. They are 47 amino acids long, have an overall positive charge and contain 8 cysteines that probably form 4 disulfide bridges characteristic of the gamma-thionins. Fabatins were active against both Gram-negative and Gram-positive bacteria, but were inactive against the yeasts Saccharomyces cerevisiae and Candida albicans.
通过酸提取法从蚕豆（Vicia faba）中分离出了两种与γ-硫堇家族相关的新型抗菌肽。提取物通过离子交换色谱进行分离，具有抗菌活性的馏分再通过反相高效液相色谱进一步纯化。对单个高效液相色谱峰的物质进行测序，结果显示存在两种仅相差一个氨基酸的肽。这些肽被命名为蚕豆素。它们长度为47个氨基酸，整体带正电荷，含有8个半胱氨酸，可能形成γ-硫堇特有的4个二硫键。蚕豆素对革兰氏阴性菌和革兰氏阳性菌均有活性，但对酿酒酵母（Saccharomyces cerevisiae）和白色念珠菌（Candida albicans）无活性。
