Toms G L, Davies J A, Woodward C G, Sweet C, Smith H
Br J Exp Pathol. 1977 Aug;58(4):444-58.
Virus titres in the nasal washes of ferrets infected with recombinant influenza virus A/PR/8/34-A/England/939/69 (H3N2) clones 7a (virulent) and 64d (attenuated) showed four phases; a phase of logarithmic increase, a plateau, a period of rapid decline and a small rise on the 4th day. The virulent clone remained in the phase of logarithmic increase longer, reached plateau levels 10-fold higher and took longer to reduce to low levels than the attenuated clone. Pyrexia (more severe and prolonged for clone 7a) and inflammatory response (similar for both clones, consisting of 90% polymorphonuclear (PMN) and 10% mononuclear (MN) phagocytes) occurred at about the same time (25-29 h after inoculation) for both clones. However, the onset of the period of rapid decline of clone 64d in the nasal washes occurred 4-8 h before that for 7a in 11 of 16 ferrets. Furthermore, significant correlations were found between increases in temperature and numbers of inflammatory cells and subsequent reductions in virus titres in nasal washes; and the delay between the responses and subsequent correlated reductions of virus was less for clone 64d than for clone 7a. Thus, a link between temperature rise and inflammatory response and subsequent reduction of virus titre was detected and, whatever the mechanisms of this link, it acted differentially on the clones of differing virulence. However, the period of logarithmic increase of clone 64d ended before pyrexia and inflammation were detected and, in the plateau stage at the onset of the host responses, there were no significant correlations between them and virus levels measured at the same time or shortly afterwards. Pyrexia and inflammation also seemed to be connected with decline of virus recoveries for infections with virus strains A/PR/8/34 (HoN1) and A/New Jersey/18/76 (Hsw15N1). The significance of the relations between pyrexia and cell response and decline of virus titres in nasal washings is discussed.
感染重组甲型流感病毒A/PR/8/34-A/England/939/69（H3N2）克隆株7a（强毒株）和64d（弱毒株）的雪貂鼻腔冲洗液中的病毒滴度呈现四个阶段：对数增长期、平台期、快速下降期以及第4天的小幅上升期。与弱毒株克隆株相比，强毒株克隆株在对数增长期持续的时间更长，达到的平台水平高10倍，且降至低水平所需的时间更长。两种克隆株的发热（7a克隆株更严重且持续时间更长）和炎症反应（两种克隆株相似，由90%的多形核（PMN）和10%的单核（MN）吞噬细胞组成）大约在同一时间（接种后25 - 29小时）出现。然而，在16只雪貂中的11只里，鼻腔冲洗液中64d克隆株快速下降期的开始时间比7a克隆株提前4 - 8小时。此外，还发现体温升高、炎症细胞数量增加与随后鼻腔冲洗液中病毒滴度降低之间存在显著相关性；64d克隆株的反应与其后病毒相关降低之间的延迟比7a克隆株短。因此，检测到体温升高、炎症反应与随后病毒滴度降低之间存在联系，无论这种联系的机制如何，它对不同毒力的克隆株作用不同。然而，64d克隆株的对数增长期在检测到发热和炎症之前就结束了，并且在宿主反应开始的平台期，它们与同时或之后不久测量的病毒水平之间没有显著相关性。发热和炎症似乎也与感染病毒株A/PR/8/34（H1N1）和A/New Jersey/18/76（Hsw1N1）时病毒回收率的下降有关。本文讨论了发热与细胞反应以及鼻腔冲洗液中病毒滴度下降之间关系的意义。
