Boyer J D, Ugen K E, Wang B, Agadjanyan M, Gilbert L, Bagarazzi M L, Chattergoon M, Frost P, Javadian A, Williams W V, Refaeli Y, Ciccarelli R B, McCallus D, Coney L, Weiner D B
Department of Pathology, University of Pennsylvania, Philadelphia 19104, USA.
Nat Med. 1997 May;3(5):526-32. doi: 10.1038/nm0597-526.
Novel approaches for the generation of more effective vaccines for HIV-1 are of significant importance. In this report we analyze the immunogenicity and efficacy of an HIV-1 DNA vaccine encoding env, rev and gag/pol in a chimpanzee model system. The immunized animals developed specific cellular and humoral immune responses. Animals were challenged with a heterologous chimpanzee titered stock of HIV-1 SF2 virus and followed for 48 weeks after challenge. Polymerase chain reaction coupled with reverse transcription (RT-PCR) results indicated infection in the control animal, whereas those animals vaccinated with the DNA constructs were protected from the establishment of infection. These studies serve as an important benchmark for the use of DNA vaccine technology for the production of protective immune responses.
开发更有效的HIV-1疫苗的新方法具有重要意义。在本报告中，我们分析了一种在黑猩猩模型系统中编码env、rev和gag/pol的HIV-1 DNA疫苗的免疫原性和效力。免疫的动物产生了特异性的细胞免疫和体液免疫反应。用异源黑猩猩滴定的HIV-1 SF2病毒毒株对动物进行攻击，并在攻击后跟踪48周。聚合酶链反应联合逆转录（RT-PCR）结果表明对照动物受到感染，而接种DNA构建体的动物则受到保护，未发生感染。这些研究为利用DNA疫苗技术产生保护性免疫反应提供了重要的基准。
