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Investigation of a pseudo-outbreak of Nocardia asteroides infection by pulsed-field gel electrophoresis and randomly amplified polymorphic DNA PCR.通过脉冲场凝胶电泳和随机扩增多态性DNA聚合酶链反应对星形诺卡菌感染的假性暴发进行调查。
J Clin Microbiol. 1997 Jun;35(6):1582-4. doi: 10.1128/jcm.35.6.1582-1584.1997.
2
Molecular typing of a suspected cluster of Nocardia farcinica infections by use of randomly amplified polymorphic DNA, pulsed-field gel electrophoresis, and amplified fragment length polymorphism analyses.运用随机扩增多态性DNA、脉冲场凝胶电泳和扩增片段长度多态性分析对疑似豚鼠诺卡菌感染聚集病例进行分子分型。
J Clin Microbiol. 2007 Dec;45(12):4048-50. doi: 10.1128/JCM.00932-07. Epub 2007 Oct 3.
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Characterization of Nocardia asteroides isolates from different ecological habitats on the basis of repetitive extragenic palindromic-PCR fingerprinting.基于重复基因外回文序列PCR指纹图谱对来自不同生态栖息地的星形诺卡菌分离株进行特征分析。
Appl Environ Microbiol. 2004 May;70(5):3149-51. doi: 10.1128/AEM.70.5.3149-3151.2004.
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Genetic relatedness analysis of nocardia strains by random amplification polymorphic Dna: validation and applications.用随机扩增多态性DNA对诺卡氏菌菌株进行遗传相关性分析：验证与应用
Res Microbiol. 2000 May;151(4):263-70. doi: 10.1016/s0923-2508(00)00152-2.
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DNA amplification and restriction endonuclease analysis for differentiation of 12 species and taxa of Nocardia, including recognition of four new taxa within the Nocardia asteroides complex.用于诺卡菌属12个种和分类单元鉴别的DNA扩增及限制性内切酶分析，包括对星状诺卡菌复合群内4个新分类单元的识别。
J Clin Microbiol. 1995 Dec;33(12):3096-101. doi: 10.1128/jcm.33.12.3096-3101.1995.
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[Nocardia asteroides].星状诺卡菌
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Rapid identification of Nocardia farcinica clinical isolates by a PCR assay targeting a 314-base-pair species-specific DNA fragment.通过针对一个314碱基对的种特异性DNA片段的聚合酶链反应（PCR）测定法快速鉴定马杜拉放线菌临床分离株。
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1
Molecular typing of a suspected cluster of Nocardia farcinica infections by use of randomly amplified polymorphic DNA, pulsed-field gel electrophoresis, and amplified fragment length polymorphism analyses.运用随机扩增多态性DNA、脉冲场凝胶电泳和扩增片段长度多态性分析对疑似豚鼠诺卡菌感染聚集病例进行分子分型。
J Clin Microbiol. 2007 Dec;45(12):4048-50. doi: 10.1128/JCM.00932-07. Epub 2007 Oct 3.
2
Clinical and laboratory features of the Nocardia spp. based on current molecular taxonomy.基于当前分子分类学的诺卡氏菌属的临床和实验室特征。
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3
Characterization of Nocardia asteroides isolates from different ecological habitats on the basis of repetitive extragenic palindromic-PCR fingerprinting.基于重复基因外回文序列PCR指纹图谱对来自不同生态栖息地的星形诺卡菌分离株进行特征分析。
Appl Environ Microbiol. 2004 May;70(5):3149-51. doi: 10.1128/AEM.70.5.3149-3151.2004.
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Use of randomly amplified polymorphic DNA as a means of developing genus- and strain-specific Streptomyces DNA probes.利用随机扩增多态性DNA技术开发属特异性和菌株特异性链霉菌DNA探针。
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6
Nocardia thyroiditis: unusual location of infection.诺卡菌性甲状腺炎：感染的罕见部位
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Molecular study of nosocomial nocardiosis outbreak involving heart transplant recipients.涉及心脏移植受者的医院内诺卡菌病暴发的分子研究。
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DNA large restriction fragment patterns of sporadic and epidemic nosocomial strains of Mycobacterium chelonae and Mycobacterium abscessus.偶发性和流行性医院内感染的龟分枝杆菌和脓肿分枝杆菌菌株的DNA大片段限制性酶切图谱
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Molecular strain typing of Mycobacterium tuberculosis to confirm cross-contamination in the mycobacteriology laboratory and modification of procedures to minimize occurrence of false-positive cultures.结核分枝杆菌的分子菌株分型，以确认分枝杆菌实验室中的交叉污染，并修改程序以尽量减少假阳性培养物的出现。
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Laboratory evaluation of an outbreak of nocardiosis in immunocompromised hosts.免疫功能低下宿主中诺卡菌病暴发的实验室评估
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Nocardia asteroides infection complicating neoplastic disease.星形诺卡菌感染并发肿瘤性疾病。
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Comparison of BACTEC 13A medium and Du Pont isolator for detection of mycobacteremia.用于检测分枝杆菌血症的BACTEC 13A培养基与杜邦隔离器的比较。
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通过脉冲场凝胶电泳和随机扩增多态性DNA聚合酶链反应对星形诺卡菌感染的假性暴发进行调查。

Investigation of a pseudo-outbreak of Nocardia asteroides infection by pulsed-field gel electrophoresis and randomly amplified polymorphic DNA PCR.

作者信息

Louie L, Louie M, Simor A E

机构信息

Department of Microbiology, Sunnybrook Health Science Centre, and University of Toronto, Ontario, Canada.

出版信息

J Clin Microbiol. 1997 Jun;35(6):1582-4. doi: 10.1128/jcm.35.6.1582-1584.1997.

DOI:10.1128/jcm.35.6.1582-1584.1997
PMID:9163487
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC229792/
Abstract

Molecular strain typing by pulsed-field gel electrophoresis and by randomly amplified polymorphic DNA analysis was used to investigate a cluster of four Nocardia asteroides isolates associated with the BACTEC 460 TB system. An instrument motor drive misalignment resulted in inadequate needle sterilization and cross-contamination of BACTEC vials. This pseudo-outbreak illustrates the importance of proper BACTEC 460 needle sterilization and maintenance and confirms the usefulness of molecular typing methods for epidemiologic investigations.

摘要

采用脉冲场凝胶电泳和随机扩增多态性DNA分析进行分子菌株分型，以研究与BACTEC 460结核系统相关的4株星形诺卡菌分离株。仪器电机驱动未对准导致BACTEC小瓶针头消毒不充分和交叉污染。这次假性暴发说明了正确进行BACTEC 460针头消毒和维护的重要性，并证实了分子分型方法在流行病学调查中的实用性。