Exmelin L, Malbruny B, Vergnaud M, Prosvost F, Boiron P, Morel C
Laboratoire de Microbiologie, Centre-Hospitalo-Universitaire Côte de Nacre, Caen, France.
J Clin Microbiol. 1996 Apr;34(4):1014-6. doi: 10.1128/jcm.34.4.1014-1016.1996.
Randomly amplified polymorphic DNA analysis and rRNA gene restriction patterns (ribotyping) were compared as methods of investigating a nosocomial outbreak of nocardiosis involving three heart transplant recipients. No clear distinctions between three clinically related isolates and four unrelated strains were obtained by ribotyping. On the contrary, randomly amplified polymorphic DNA analysis with two selected primers, primers 2650 and DKU49, showed one pattern for the three related isolates and four patterns for the unrelated strains.
作为调查一起涉及三名心脏移植受者的医院内诺卡菌病暴发的方法,对随机扩增多态性DNA分析和rRNA基因限制性图谱(核糖体分型)进行了比较。通过核糖体分型,在三个临床相关分离株和四个不相关菌株之间未获得明确区分。相反,使用两种选定引物(引物2650和DKU49)进行的随机扩增多态性DNA分析显示,三个相关分离株呈现一种图谱,而不相关菌株呈现四种图谱。