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Comparison of phage typing and DNA fingerprinting by polymerase chain reaction for discrimination of methicillin-resistant Staphylococcus aureus strains.通过聚合酶链反应进行噬菌体分型和DNA指纹分析以鉴别耐甲氧西林金黄色葡萄球菌菌株的比较
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DNA fragment length polymorphism analysis of Mycobacterium tuberculosis isolates by arbitrarily primed polymerase chain reaction.应用随机引物聚合酶链反应对结核分枝杆菌分离株进行DNA片段长度多态性分析。
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The medically important aerobic actinomycetes: epidemiology and microbiology.具有医学重要性的需氧放线菌：流行病学与微生物学
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Laboratory evaluation of an outbreak of nocardiosis in immunocompromised hosts.免疫功能低下宿主中诺卡菌病暴发的实验室评估
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涉及心脏移植受者的医院内诺卡菌病暴发的分子研究。

Molecular study of nosocomial nocardiosis outbreak involving heart transplant recipients.

作者信息

Exmelin L, Malbruny B, Vergnaud M, Prosvost F, Boiron P, Morel C

机构信息

Laboratoire de Microbiologie, Centre-Hospitalo-Universitaire Côte de Nacre, Caen, France.

出版信息

J Clin Microbiol. 1996 Apr;34(4):1014-6. doi: 10.1128/jcm.34.4.1014-1016.1996.

DOI:10.1128/jcm.34.4.1014-1016.1996
PMID:8815073
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC228943/
Abstract

Randomly amplified polymorphic DNA analysis and rRNA gene restriction patterns (ribotyping) were compared as methods of investigating a nosocomial outbreak of nocardiosis involving three heart transplant recipients. No clear distinctions between three clinically related isolates and four unrelated strains were obtained by ribotyping. On the contrary, randomly amplified polymorphic DNA analysis with two selected primers, primers 2650 and DKU49, showed one pattern for the three related isolates and four patterns for the unrelated strains.

摘要

作为调查一起涉及三名心脏移植受者的医院内诺卡菌病暴发的方法，对随机扩增多态性DNA分析和rRNA基因限制性图谱（核糖体分型）进行了比较。通过核糖体分型，在三个临床相关分离株和四个不相关菌株之间未获得明确区分。相反，使用两种选定引物（引物2650和DKU49）进行的随机扩增多态性DNA分析显示，三个相关分离株呈现一种图谱，而不相关菌株呈现四种图谱。