Suter T M, Viswanathan V K, Cianciotto N P
Department of Microbiology-Immunology, Northwestern University, Chicago, Illinois 60611, USA.
Antimicrob Agents Chemother. 1997 Jun;41(6):1385-8. doi: 10.1128/AAC.41.6.1385.
A gene capable of conferring spectinomycin resistance was isolated from Legionella pneumophila, the agent of Legionnaires' disease. The gene (aph) encoded a 36-kDa protein which has similarity to aminoglycoside phosphotransferases. Biochemical analysis confirmed that aph encodes a phosphotransferase which modifies spectinomycin but not hygromycin, kanamycin, or streptomycin. The strain that was the source of aph demonstrated resistance to spectinomycin, and Southern hybridizations determined that aph also exists in other legionellae.
从军团病病原体嗜肺军团菌中分离出一种能够赋予壮观霉素抗性的基因。该基因（aph）编码一种36 kDa的蛋白质，与氨基糖苷磷酸转移酶具有相似性。生化分析证实，aph编码一种磷酸转移酶，该酶可修饰壮观霉素，但不能修饰潮霉素、卡那霉素或链霉素。aph的来源菌株对壮观霉素具有抗性，Southern杂交确定aph也存在于其他军团菌中。
