Kishida T, Wang W, Fukuda M, Tamaki Y
Department of Forensic Medicine, Oita Medical University, Japan.
Nihon Hoigaku Zasshi. 1997 Apr;51(2):67-9.
Using a duplex polymerase chain reaction (PCR) system, we successfully amplified GATA microsatellite loci Y-27H39 and HPRT on the Y and X chromosomes, respectively, and detected their alleles with a digoxigenin-labeled (GATA)6 probe. Among 450 unrelated Japanese including 225 females, we found 6 HPRT alleles ranging from 155 to 175 nucleotides with a simple repeat structure comprising 11 to 16 repeats. The duplex PCR is highly informative in Japanese.
我们使用双重聚合酶链反应（PCR）系统，分别成功扩增了Y染色体上的GATA微卫星位点Y-27H39和X染色体上的次黄嘌呤磷酸核糖转移酶（HPRT），并用洋地黄毒苷标记的（GATA）6探针检测了它们的等位基因。在包括225名女性在内的450名无关日本人群体中，我们发现了6个HPRT等位基因，长度在155至175个核苷酸之间，具有由11至16个重复序列组成的简单重复结构。双重PCR在日本人群体中具有高度的信息性。
