Shichi H, Page T, Sahouri M J, Shin D H
Department of Ophthalmology, Kresge Eye Institute, Wayne State University, Detroit, Michigan, USA.
J Ocul Pharmacol Ther. 1997 Jun;13(3):201-6. doi: 10.1089/jop.1997.13.201.
An inexpensive microplate assay method was developed to determine ascorbic acid in human aqueous humor samples. The method is based on the selective oxidation of ascorbic acid at alkaline pH and determination of ascorbic acid and other reducing substances in samples with bicinchoninic acid/CuSO4 before and after the alkaline treatment. The two-point measurement eliminates the effect of interfering substances, such as glucose, uric acid and glutathione, which are stable at alkaline pH. The advantages of the method are that it requires small sample volumes and allows handling of a large number of samples simultaneously.
开发了一种廉价的微孔板检测方法来测定人房水样本中的抗坏血酸。该方法基于在碱性pH条件下抗坏血酸的选择性氧化，并在碱性处理前后用二辛可宁酸/CuSO4测定样本中的抗坏血酸和其他还原物质。两点测量消除了干扰物质的影响，如葡萄糖、尿酸和谷胱甘肽，它们在碱性pH条件下是稳定的。该方法的优点是所需样本量小，并且允许同时处理大量样本。
