Daisy J A, Lief F S, Friedman H M
J Clin Microbiol. 1979 Jun;9(6):688-92. doi: 10.1128/jcm.9.6.688-692.1979.
The efficacy for direct immunofluorescence of a commercial conjugate for influenza A virus prepared against whole A/Udorn (H3NS) virus was studied. The conjugate was specific for influenza A virus, but its sensitivity varied depending upon the strain of influenza A tested. Nasopharyngeal aspirates collected from 25 patients during an outbreak of influenza were examined for viral antigen with the conjugates and inoculated onto monkey kidney (MK) cells for virus isolation. Fifteen patients had isolates for influenza A/USSR/90/77 (H1N1); nasopharyngeal secretions were fluorescent antibody positive in 12. Fluorescent antibody was copositive with culture in 11/15 patients (73.3%) and conegative in 9/10 (90%). The one fluorescent antibody-positive, culture-negative patient had negative serology for influenza A and the fluorescent antibody result was considered to be a false positive. At a 1:10 dilution, the conjugate stained nasopharyngeal and MK cells infected with A/USSR (H1N1) 2 to 3+, whereas cells infected with H3N2 virus stained 4+. A conjugate made specifically against the ribonucleoprotein antigen, which is universal to all influenza A strains, may improve the sensitivity of the direct immunofluorescent test.
对一种针对全株A/Udorn（H3N2）病毒制备的甲型流感病毒商业偶联物进行了直接免疫荧光检测的效果研究。该偶联物对甲型流感病毒具有特异性，但其敏感性因所检测的甲型流感病毒株而异。在一次流感暴发期间，从25名患者采集的鼻咽抽吸物用该偶联物检测病毒抗原，并接种到猴肾（MK）细胞上进行病毒分离。15名患者分离出甲型流感病毒/苏联/90/77（H1N1）；12例患者的鼻咽分泌物荧光抗体呈阳性。荧光抗体在11/15例患者（73.3%）中与培养结果呈阳性，在9/10例患者（90%）中呈阴性。1例荧光抗体阳性、培养阴性的患者甲型流感病毒血清学检测为阴性，荧光抗体结果被认为是假阳性。在1:10稀释度下，该偶联物对感染A/苏联（H1N1）的鼻咽和MK细胞染色为2至3+，而感染H3N2病毒的细胞染色为4+。一种针对所有甲型流感病毒株普遍存在的核糖核蛋白抗原特异性制备的偶联物，可能会提高直接免疫荧光检测的敏感性。
