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条件培养基促进非允许性宿主巨噬细胞中的利什曼原虫感染。

Promotion of leishmanial infections in non-permissive host macrophages by conditioned medium.

作者信息

Handman E, Greenblatt C L

出版信息

Z Parasitenkd. 1977 Sep 21;53(2):143-7. doi: 10.1007/BF00380458.

DOI:10.1007/BF00380458
PMID:919693
Abstract

Leishmania enriettii grows well in guinea pig macrophages in culture but fails to do so in mouse macrophages. Dialyzed and concentrated conditioned medium from L. enriettii cultures promoted the infection in the mouse macrophages at 37 degrees C and at 32 degrees C. Similarly processed medium on which L. tropica had been cultured did not cause the same effect. Immune precipitation of the conditioned medium by anti-Leishmania serum cancelled out the effect. Infections of guinea pig macrophages by L. tropica were also enhanced by the homologous medium, but we were unable to quantitate the effect.

摘要

恩氏利什曼原虫在培养的豚鼠巨噬细胞中生长良好,但在小鼠巨噬细胞中则不然。恩氏利什曼原虫培养物经透析和浓缩的条件培养基在37摄氏度和32摄氏度下可促进对小鼠巨噬细胞的感染。用同样方法处理过的、曾培养过热带利什曼原虫的培养基则不会产生同样的效果。抗利什曼原虫血清对条件培养基的免疫沉淀消除了这种效果。热带利什曼原虫对豚鼠巨噬细胞的感染也会因同源培养基而增强,但我们无法对这种效果进行定量。

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本文引用的文献

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Response of cultured macrophages to Mycobacterium tuberculosis, with observations on fusion of lysosomes with phagosomes.培养的巨噬细胞对结核分枝杆菌的反应,以及关于溶酶体与吞噬体融合的观察。
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Immunity in cutaneous leishmaniasis of the guinea-pig.豚鼠皮肤利什曼病的免疫
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