Liu B, Hu R, Deng J
Department of Chemistry, Fuden University, Shanghai, People's Republic of China.
Anal Chem. 1997 Jul 1;69(13):2343-8. doi: 10.1021/ac960930u.
A new approach to construct an amperometric biosensor is described. Without using bovine serum albumin-glutaraldehyde, glucose oxidase (GOx) was immobilized on a dealuminized Y zeolite (DAY)-modified platinum electrode to construct a glucose sensor. The large specific surface area of the zeolite substrate resulted in high enzyme loading. The immobilized GOx in this manner was stable and could maintain its high activity for at least 3 months. The interactions between the zeolite and the enzyme were investigated by means of Fourier transform infrared spectra, and the pore distribution and the surface acid property of DAY were preliminarily studied. The results showed that the hydrophilic property and the existing mesopores of DAY played important roles in the enzyme immobilization. This resulting biosensor exhibited good reproducibility and selectivity, owing to the uniform pore structure and unique ion-exchange property of the zeolite. The biosensor responded rapidly to glucose in the linear range from 2.0 x 10(-6) to 3.0 x 10(-3) M, with a detection limit of 0.5 microM.
描述了一种构建电流型生物传感器的新方法。在不使用牛血清白蛋白 - 戊二醛的情况下，将葡萄糖氧化酶（GOx）固定在脱铝Y型沸石（DAY）修饰的铂电极上以构建葡萄糖传感器。沸石底物的大比表面积导致高酶负载量。以这种方式固定的GOx是稳定的，并且可以保持其高活性至少3个月。通过傅里叶变换红外光谱研究了沸石与酶之间的相互作用，并初步研究了DAY的孔分布和表面酸性。结果表明，DAY的亲水性和现有的介孔在酶固定化中起重要作用。由于沸石均匀的孔结构和独特的离子交换性能，所得生物传感器表现出良好的重现性和选择性。该生物传感器在2.0×10（-6）至3.0×10（-3）M的线性范围内对葡萄糖快速响应，检测限为0.5 microM。
