Iwaya F, Igari T, Hoshino S, Hikichi H
Department of Cardiovascular Surgery, Fukushima Medical College, Japan.
Artif Organs. 1997 Jul;21(7):700-3. doi: 10.1111/j.1525-1594.1997.tb03726.x.
To evaluate the blood trauma caused by a new device for producing a pulsatile flow of the centrifugal pump, the pulsatile assist device for the centrifugal pump (PAD-CP) that we have developed, a hemolysis study was performed in vitro and in animal experimentation. For the in vitro testing, 2 identical sets of hemolysis test circuits were prepared with 2,400 ml of bovine blood. The 2 circuits were pumped simultaneously. Plasma total hemoglobin levels were less than 40 mg/dl after 3 h, under a pump flow of 2 L/min. Hemolysis increased to a severe level after 4 h of 4 L/min pump flow. The cause of this hemolysis was thought to be a vibration of the circuit because of incomplete compression of the polyurethane tube in the PAD-CP. Five adult sheep (average body weight, 47 kg) were used for in vivo evaluation of hemolysis. Hemolysis was less than 30 mg/dl of plasma hemoglobin after 4 h of open chest extracorporeal circulation with 3.0-3.6 L/min of flow rate using the PAD-CP. Other hematologic changes after PAD-CP driving were within normal limits. We conclude that the PAD-CP has proven to have possible clinical applications.
为评估一种用于产生离心泵脉动流的新装置——我们研发的离心泵脉动辅助装置（PAD-CP）所导致的血液损伤，我们进行了体外和动物实验的溶血研究。对于体外测试，用2400毫升牛血制备了2套相同的溶血测试回路。2个回路同时进行泵血。在泵流量为2升/分钟的情况下，3小时后血浆总血红蛋白水平低于40毫克/分升。在泵流量为4升/分钟的情况下，4小时后溶血增加到严重程度。这种溶血的原因被认为是由于PAD-CP中聚氨酯管压缩不完全导致回路振动。5只成年绵羊（平均体重47千克）用于溶血的体内评估。使用PAD-CP在体外循环开胸4小时、流速为3.0 - 3.6升/分钟的情况下，血浆血红蛋白溶血低于30毫克/分升。PAD-CP驱动后的其他血液学变化在正常范围内。我们得出结论，PAD-CP已被证明具有可能的临床应用价值。
