Else K J, Hültner L, Grencis R K
Department of Cell and Structural Biology, University of Manchester, U.K.
Immunology. 1992 Feb;75(2):232-7.
We have analysed the production of a wide variety of cytokines by in vitro concanavalin A (Con A) stimulated mesenteric lymph node cells (MLNC) from strains of mice experiencing chronic (B10.BR, AKR) versus acute (BALB/K) infection with the nematode parasite Trichuris muris. MLNC from infected BALB/K mice produced elevated levels of the Th2-specific cytokines interleukin-5 (IL-5) and IL-9. IL-3 and IL-4 remained at or just above normal. Interferon-gamma (IFN-gamma) (a Th1-type cytokine) was secreted only in small amounts. MLNC from infected susceptible B10.BR and AKR mice produced large amounts of IFN-gamma in the relative absence of IL-4 and IL-9. IL-5 levels failed to rise significantly above normal in B10.BR mice whilst in AKR mice high levels of IL-5 were detected early post-infection (p.i.) but levels decreased dramatically as the infection proceeded to reach normal levels by Day 34. IL-3 levels were depressed below normal. Our results are consistent with the polarization of the Th-cell response during T. muris infection to give predominantly IFN-gamma-secreting Th1 cells in strains of mice unable to expel the parasite and mainly IL-4, IL-5 and IL-9 producing Th2-type cells in resistant strains.
我们分析了用体外伴刀豆球蛋白A（Con A）刺激来自感染线虫寄生虫毛首鞭形线虫的慢性感染（B10.BR、AKR）与急性感染（BALB/K）小鼠品系的肠系膜淋巴结细胞（MLNC）所产生的多种细胞因子。来自感染BALB/K小鼠的MLNC产生了升高水平的Th2特异性细胞因子白细胞介素-5（IL-5）和IL-9。IL-3和IL-4保持在正常水平或略高于正常水平。干扰素-γ（IFN-γ）（一种Th1型细胞因子）仅少量分泌。来自感染易感B10.BR和AKR小鼠的MLNC在相对缺乏IL-4和IL-9的情况下产生大量IFN-γ。在B10.BR小鼠中，IL-5水平未能显著高于正常水平，而在AKR小鼠中，感染后早期（p.i.）检测到高水平的IL-5，但随着感染进展，到第34天时水平急剧下降至正常水平。IL-3水平低于正常水平。我们的结果与毛首鞭形线虫感染期间Th细胞反应的极化一致，即在无法驱除寄生虫的小鼠品系中主要产生分泌IFN-γ的Th1细胞，而在抗性品系中主要产生IL-4、IL-5和IL-9的Th2型细胞。
