Sevick-Muraca E M, Lopez G, Reynolds J S, Troy T L, Hutchinson C L
School of Chemical Engineering, Purdue University, West Lafayette, IN, USA.
Photochem Photobiol. 1997 Jul;66(1):55-64. doi: 10.1111/j.1751-1097.1997.tb03138.x.
The ability to optically image or detect diseased tissue volumes located deep within tissues depends upon the degree of contrast provided by differences in local optical properties. In this report, we show that the exogenous contrast offered by fluorescent compounds is superior to that provided by nonfluorescing, light-absorbing compounds when time-dependent measurements are employed. In addition, we show that the induced contrast is not only moderated by the preferential uptake of fluorescent agents into diseased tissue volumes of interest but also by the fluorescent optical properties and the fluorescence dynamics in the specific tissue volume. Using tissue phantom studies, we demonstrated experimentally that near-infrared-absorbing and fluorescent dyes such as indocyanine green can provide detection of diseased tissue volumes from fluorescence measurements made at the periphery of tissue when there is perfect, 100-fold and 10-fold partitioning in diseased tissues over that in surrounding normal tissues. Experimental results of common laser dyes show the contrast is also mediated by the quantum yield and lifetime parameters that may be dependent upon the local tissue environment.
对位于组织深处的患病组织体积进行光学成像或检测的能力取决于局部光学特性差异所提供的对比度。在本报告中，我们表明，当采用随时间变化的测量方法时，荧光化合物提供的外源性对比度优于非荧光、吸光化合物提供的对比度。此外，我们表明，诱导对比度不仅受荧光剂优先摄取到感兴趣的患病组织体积中的影响，还受特定组织体积中的荧光光学特性和荧光动力学的影响。通过组织模型研究，我们通过实验证明，当患病组织与周围正常组织的分配比例达到完美的100倍和10倍时，近红外吸收和荧光染料（如吲哚菁绿）可以通过在组织周边进行荧光测量来检测患病组织体积。常见激光染料的实验结果表明，对比度还受量子产率和寿命参数的调节，这些参数可能取决于局部组织环境。
