Everbach E C, Makin I R, Azadniv M, Meltzer R S
Department of Engineering, Swarthmore College, PA 19081-1397, USA.
Ultrasound Med Biol. 1997;23(4):619-24. doi: 10.1016/s0301-5629(97)00039-2.
A 20-MHz passive acoustic detector was used to quantify the amount of transient acoustic cavitation occurring in a sample exposed to intense pulsed ultrasound. A dilute suspension of human erythrocytes with and without a microbubble echo-contrast agent was exposed in vitro to 500 W/cm2 (SPPA) ultrasound of center frequency 1 MHz and tone burst duration 20, 100, 200, 500 and 1000 microseconds at a pulse repetition frequency of 20 Hz. Inertial cavitation occurring within the sample, as measured by the temporal average of the detector output, correlated well with hemolysis, suggesting that violent bubble collapse is responsible for cell damage. The result also raises the prospect of cavitation monitoring as a possible predictor of adverse bioeffects when echo-contrast agents are used clinically.
使用一个20兆赫兹的无源声学探测器来量化暴露于高强度脉冲超声的样品中发生的瞬态声空化的量。含有和不含有微泡回声造影剂的人红细胞稀释悬浮液在体外暴露于中心频率为1兆赫兹、脉冲串持续时间为20、100、200、500和1000微秒、脉冲重复频率为20赫兹、声压峰值为500瓦/平方厘米的超声下。通过探测器输出的时间平均值测量，样品内发生的惯性空化与溶血密切相关，这表明剧烈的气泡破裂是细胞损伤的原因。该结果还提出了在临床使用回声造影剂时，将空化监测作为不良生物效应可能预测指标的前景。
