Everbach E C, Makin I R, Francis C W, Meltzer R S
Department of Engineering, Swarthmore College, PA 19081-1397, USA.
Ultrasound Med Biol. 1998 Jan;24(1):129-36. doi: 10.1016/s0301-5629(97)00233-0.
A suspension of human platelets in autologous plasma or buffer solution with and without a microbubble echo-contrast agent was exposed in vitro to 730 W/cm2 (ISPPA) ultrasound pulses of duration 40-160 microseconds at 1 MHz and 20-Hz pulse repetition frequency. Inertial cavitation occurring within the samples was monitored during the exposures and a measure of average cavitational activity was calculated for each 5-min exposure. This quantity, with the other acoustic parameters, accounted for up to 75% of the variation in the destruction of platelets as measured by Coulter counter and 83.5% of the release of bound radiolabel using a multiple-interaction statistical model. When the echo-contrast agent was absent, negligible cavitation occurred and the amount of platelet destruction was statistically indistinguishable from sham (no-ultrasound) exposures. Therefore, microbubble echo-contrast agents may interact with ultrasound to cause platelet lysis through the mechanism of inertial cavitation.
将含有和不含有微泡回声造影剂的人血小板悬浮于自体血浆或缓冲溶液中，在体外将其暴露于频率为1MHz、脉冲重复频率为20Hz、持续时间为40 - 160微秒、强度为730W/cm²（空间峰值脉冲平均强度）的超声脉冲下。在暴露过程中监测样品内发生的惯性空化，并针对每次5分钟的暴露计算平均空化活性的量度。利用多相互作用统计模型，该量与其他声学参数一起，在通过库尔特计数器测量的血小板破坏变化中占比高达75%，在结合放射性标记物的释放中占比83.5%。当不存在回声造影剂时，发生的空化可忽略不计，并且血小板破坏量在统计学上与假暴露（无超声）无差异。因此，微泡回声造影剂可能与超声相互作用，通过惯性空化机制导致血小板裂解。
