Wittekind D, Hilgarth M, Kretschmer V
Geburtshilfe Frauenheilkd. 1979 Nov;39(11):969-72.
A simple and well reproducable modification of the Papanicolaou stain is reported. The most important modification is the replacement of the natural stain hematoxyline by the qualitively excellent synthetic stain thionin which is immediately added to the alcoholic fixation solution for a fixation stain. The high labor intense alternation between alcohol water and alcohol is thus eliminated. The quality of the nuclear staining is very good. The fixation staining with thionin without counter-staining of the cytoplasma may be used for a rapid thionin stain which gives sufficient cytoplasmatic staining by the thionin for a rapid cytological smear for cancer. The counter-stain of the cytoplasma was also simplified. The over-all staining of this simplification of the pap smear is comparable to that of the original papanicolaou stain. This modification of the papanicolaou stain fulfills requirements which a stain in competition with the original papanicolaou stain should have. 1. The modification is qualitively equal to the original method, 2. the staining method is simpler, 3. the staining method is well reproducable, 4. the staining method is more economical.
本文报道了一种简单且可重复性良好的巴氏染色改良方法。最重要的改良是用质量优异的合成染料硫堇替代天然染料苏木精，硫堇直接添加到酒精固定液中用于固定染色。这样就消除了酒精与水之间高强度的人工交替步骤。细胞核染色质量非常好。用硫堇进行固定染色而不进行细胞质复染，可用于快速硫堇染色，该方法通过硫堇对细胞质进行充分染色，适用于癌症快速细胞学涂片。细胞质复染也得到了简化。这种巴氏涂片简化染色法的整体染色效果与原始巴氏染色相当。这种巴氏染色的改良方法满足了与原始巴氏染色竞争的染色方法应具备的要求。1. 改良在质量上与原始方法相当；2. 染色方法更简单；3. 染色方法可重复性良好；4. 染色方法更经济。
