Ota H, Katsuyama T
Department of Laboratory Medicine, Shinshu University School of Medicine, Matsumoto, Japan.
Histochem J. 1992 Feb;24(2):86-92. doi: 10.1007/BF01082444.
Attempts have been made to develop a procedure for preserving and analysing the surface mucous layer of the human stomach in paraffin sections. Histologically normal gastric mucosae were obtained from 20 surgically removed stomachs. Of the different fixatives tested, Carnoy's solution gave rise to the most satisfactory results. In Haematoxylin-Eosin stained sections, the surface mucous layer appeared as a thick eosinophilic layer coating the gastric mucosal surface and measured 55.4 +/- 2.5 microns in the fundus and 21.8 +/- 1.0 microns in the pylorus respectively. A dual staining method consisting of galactose oxidase-cold thionine Schiff and paradoxical concanavalin A staining was applied to the surface mucous layer in order to reveal the distribution pattern of mucins secreted by two types of mucous cell in the gastric mucosa: surface mucous cells and gland mucous cells. As a result of this staining, an alternating laminated layer was visualized which consisted of the particular two types of mucin. In five cases, the surface mucous layer was examined in unfixed frozen sections. This layer was only partially preserved but revealed the same laminated structure. These results indicated that gland mucous cell mucins contribute to form the surface mucous layer.
人们已尝试开发一种用于在石蜡切片中保存和分析人胃表面黏液层的方法。从20个手术切除的胃中获取了组织学正常的胃黏膜。在测试的不同固定剂中，卡诺氏固定液产生的结果最令人满意。在苏木精-伊红染色切片中，表面黏液层表现为覆盖胃黏膜表面的一层厚厚的嗜酸性层，在胃底分别为55.4±2.5微米，在幽门为21.8±1.0微米。为了揭示胃黏膜中两种黏液细胞（表面黏液细胞和腺黏液细胞）分泌的黏蛋白的分布模式，对表面黏液层采用了半乳糖氧化酶-冷硫堇席夫染色和反常伴刀豆球蛋白A染色的双重染色方法。经过这种染色，可见到由特定的两种黏蛋白组成的交替层状结构。在5例中，对未固定的冰冻切片中的表面黏液层进行了检查。该层仅部分保存，但显示出相同的层状结构。这些结果表明腺黏液细胞黏蛋白有助于形成表面黏液层。
