使用科玛嘉念珠菌显色培养基从牙科样本中分离酵母菌。
Use of CHROMagar Candida medium for isolation of yeasts from dental samples.
作者信息
Beighton D, Ludford R, Clark D T, Brailsford S R, Pankhurst C L, Tinsley G F, Fiske J, Lewis D, Daly B, Khalifa N
机构信息
RCS Department of Dental Sciences, King's College School of Medicine and Dentistry, London, United Kingdom.
出版信息
J Clin Microbiol. 1995 Nov;33(11):3025-7. doi: 10.1128/jcm.33.11.3025-3027.1995.
Abstract
A new differential medium, CHROMagar Candida, for the isolation of clinically important yeasts was investigated to determine its usefulness in facilitating the study of oral yeasts. The recovery of yeasts on the medium was not significantly different from the recovery on Sabouraud dextrose agar. The identities of 450 green colonies on CHROMagar Candida, presumptively identified as Candida albicans on the basis of the manufacturer's instructions, were confirmed by testing for beta-N-acetylgalactosaminidase. Candida tropicalis also formed distinctive colonies, and other yeasts including Candida (Torulopsis) glabrata, Candida Parapsilosis, Candida Magnoliae, Candida lusitaniae, Candida Famata, Candida kefir, and Saccharomyces cerevisiae were readily distinguished from C. albicans and C. tropicalis isolates. CHROMagar Candida is a very useful medium, and its use will facilitate the study of yeasts associated with dental diseases.
摘要
一种用于分离临床上重要酵母菌的新型鉴别培养基——科玛嘉念珠菌显色培养基，被用于研究其在促进口腔酵母菌研究方面的实用性。该培养基上酵母菌的回收率与沙氏葡萄糖琼脂上的回收率无显著差异。通过检测β-N-乙酰半乳糖胺酶，证实了科玛嘉念珠菌显色培养基上450个绿色菌落的身份，这些菌落在制造商的说明基础上被初步鉴定为白色念珠菌。热带念珠菌也形成了独特的菌落，包括光滑念珠菌、近平滑念珠菌、季也蒙念珠菌、葡萄牙念珠菌、法塔念珠菌、高加索念珠菌和酿酒酵母在内的其他酵母菌很容易与白色念珠菌和热带念珠菌分离株区分开来。科玛嘉念珠菌显色培养基是一种非常有用的培养基，它的使用将有助于研究与牙齿疾病相关的酵母菌。
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本文引用的文献
1
EVALUATION OF A MOLYBDENUM CULTURE MEDIUM AS SELECTIVE AND DIFFERENTIAL FOR YEASTS.一种钼培养基对酵母的选择性及鉴别性评估
J Pathol Bacteriol. 1964 Apr;87:428-31. doi: 10.1002/path.1700870226.
2
Diagnostic medium for differentiation of species of Candida.用于区分念珠菌属物种的诊断培养基。
Antibiot Annu. 1957;5:137-43.
3
CHROMagar Candida, a new differential isolation medium for presumptive identification of clinically important Candida species.科玛嘉念珠菌显色培养基，一种用于初步鉴定临床重要念珠菌属菌种的新型鉴别分离培养基。
J Clin Microbiol. 1994 Aug;32(8):1923-9. doi: 10.1128/jcm.32.8.1923-1929.1994.
4
Rapid identification of Candida albicans by using Albicans ID and fluoroplate agar plates.使用白色念珠菌鉴定试剂盒和荧光平板琼脂平板快速鉴定白色念珠菌。
J Clin Microbiol. 1994 Dec;32(12):3034-6. doi: 10.1128/jcm.32.12.3034-3036.1994.
5
The relation of stimulated salivary flow rate and pH to Lactobacillus and yeast concentrations in saliva.刺激唾液流速及pH值与唾液中乳酸杆菌和酵母菌浓度的关系。
J Dent Res. 1981 Dec;60(12):1929-35. doi: 10.1177/00220345810600120201.
6
Umbelliferyl-labeled galactosaminide as an aid in identification of Candida albicans.伞形花内酯标记的半乳糖胺作为鉴定白色念珠菌的辅助手段。
J Clin Microbiol. 1987 Dec;25(12):2424-5. doi: 10.1128/jcm.25.12.2424-2425.1987.
7
A comparison of oral rinse and imprint sampling techniques for the detection of yeast, coliform and Staphylococcus aureus carriage in the oral cavity.口腔冲洗和印记取样技术在检测口腔中酵母菌、大肠菌群和金黄色葡萄球菌携带情况方面的比较。
J Oral Pathol. 1986 Aug;15(7):386-8. doi: 10.1111/j.1600-0714.1986.tb00646.x.
8
Denture stomatitis in the elderly.老年人义齿性口炎
Oral Microbiol Immunol. 1990 Apr;5(2):82-5. doi: 10.1111/j.1399-302x.1990.tb00232.x.
9
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10
Salivary levels of mutans streptococci, lactobacilli, yeasts, and root caries prevalence in non-institutionalized elderly dental patients.非机构养老老年牙科患者的唾液变形链球菌、乳酸菌、酵母菌水平及根龋患病率
Community Dent Oral Epidemiol. 1991 Oct;19(5):302-7. doi: 10.1111/j.1600-0528.1991.tb00172.x.
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