Rohde T, MacLean D A, Richter E A, Kiens B, Pedersen B K
Copenhagen Muscle Research Centre, Department of Infectious Diseases, Rigshospitalet, Copenhagen N, Denmark.
Am J Physiol. 1997 Jul;273(1 Pt 1):E85-91. doi: 10.1152/ajpendo.1997.273.1.E85.
To study the relationship between exercise-related muscle proteolysis and the cytokine response, a prolonged eccentric exercise model of one leg was used. Subjects performed two trials [a branched-chain amino acid (BCAA) supplementation and a control trial]. The release of amino acids from muscle during and after the eccentric exercise was decreased in the BCAA trial, suggesting a suppression of net muscle protein degradation. The plasma concentrations of interleukin (IL)-6 increased from 0.75 +/- 0.19 (preexercise) to 5.02 +/- 0.96 pg/ml (2 h postexercise) in the control trial and in the BCAA supplementation trial from 1.07 +/- 0.41 to 4.15 +/- 1.21 pg/ml. Eccentric exercise had no effect on the concentrations of neutrophils, lymphocytes, CD16+/CD56+, CD4+, CD8+, CD14+/CD38+, lymphocyte proliferative response, or cytotoxic activities. BCAA supplementation reduced the concentration of CD14+/CD38+ cells. This study shows that the concentration of IL-6 in plasma is increased after prolonged eccentric exercise and suggests that the cytokine response is independent of the muscle proteolysis that occur during exercise.
为研究运动相关的肌肉蛋白水解与细胞因子反应之间的关系，采用了单腿长时间离心运动模型。受试者进行了两项试验[支链氨基酸（BCAA）补充试验和对照试验]。在BCAA试验中，离心运动期间及之后肌肉中氨基酸的释放减少，提示净肌肉蛋白降解受到抑制。在对照试验中，血浆白细胞介素（IL）-6浓度从0.75±0.19（运动前）增加至5.02±0.96 pg/ml（运动后2小时），在BCAA补充试验中从1.07±0.41增加至4.15±1.21 pg/ml。离心运动对中性粒细胞、淋巴细胞、CD16+/CD56+、CD4+、CD8+、CD14+/CD38+的浓度、淋巴细胞增殖反应或细胞毒性活性均无影响。补充BCAA降低了CD14+/CD38+细胞的浓度。本研究表明，长时间离心运动后血浆中IL-6浓度升高，并提示细胞因子反应独立于运动期间发生的肌肉蛋白水解。
