Mukherjee S, Crawford J M, McClear N, Tsang A
Department of Periodontics, University of Illinois, Chicago 60612-7212, USA.
Biol Trace Elem Res. 1997 Apr;57(1):1-8. doi: 10.1007/BF02803864.
Availability of iron is one important nutritional parameter for microbial growth in saliva. This longitudinal study measured the diurnal and day-to-day variations in the total iron (TI), total iron-binding capacity (TIBC), unsaturated iron-binding capacity (UIBC), and lactoferrin (LF) in unstimulated human parotid saliva. Saliva was collected from 15 young male subjects in the morning and afternoon hours each day for five consecutive days. The TI and TIBC were determined by flameless atomic absorption spectroscopy, and UIBC was determined by subtraction of TI from TIBC. The LF was determined by "sandwich" enzyme-linked immunosorbent assay (ELISA). One peripheral blood sample of each subject was also analyzed for TI, TIBC, and ferritin. The results showed no significant diurnal or day-to-day variation of TI, TIBC, UIBC, or LF in saliva for most subjects. However, significant between-subject variations were observed for most parameters. Variations ranged from subjects with constantly positive UIBC values to subjects with constantly negative UIBC values. The relationship between the LF values and the TI and TIBC values suggests that other iron-binding protein(s) are present in saliva. Also, saliva had significantly lower TIBC values than serum. This finding indicated that iron may be easily available in saliva. However, further studies are required to determine the relationship between UIBC value of saliva and oral and dental diseases, and also to detect the presence of other iron-binding proteins in saliva.
铁的可利用性是唾液中微生物生长的一个重要营养参数。这项纵向研究测量了未刺激的人腮腺唾液中总铁（TI）、总铁结合力（TIBC）、不饱和铁结合力（UIBC）和乳铁蛋白（LF）的昼夜及每日变化。连续五天每天上午和下午从15名年轻男性受试者中收集唾液。TI和TIBC通过无火焰原子吸收光谱法测定，UIBC通过从TIBC中减去TI来测定。LF通过“夹心”酶联免疫吸附测定法（ELISA）测定。还对每个受试者的一份外周血样本进行了TI、TIBC和铁蛋白分析。结果显示，大多数受试者唾液中的TI、TIBC、UIBC或LF没有显著的昼夜或每日变化。然而，大多数参数观察到了显著的个体间差异。差异范围从UIBC值持续为正的受试者到UIBC值持续为负的受试者。LF值与TI和TIBC值之间的关系表明唾液中存在其他铁结合蛋白。此外，唾液的TIBC值明显低于血清。这一发现表明唾液中的铁可能易于利用。然而，需要进一步研究以确定唾液的UIBC值与口腔和牙齿疾病之间的关系，以及检测唾液中其他铁结合蛋白的存在。
