Mustapha A, Jiang T, Savaiano D A
Department of Food Science and Human Nutrition, University of Missouri, Columbia 65211, USA.
J Dairy Sci. 1997 Aug;80(8):1537-45. doi: 10.3168/jds.S0022-0302(97)76083-1.
The influence of bile sensitivity, lactose transport, and acid tolerance of Lactobacillus acidophilus on in vivo digestion of lactose was investigated. Four strains of L. acidophilus exhibiting varied degrees of lactose transport, beta-galactosidase activity, and bile sensitivity were used to prepare unfermented acidophilus milks. Lactose malabsorption was evaluated by measuring breath H2 excretion off 11 lactose maldigesting subjects following ingestion of four acidophilus test milks. Test meals were fed in a randomized double-blind protocol. Consumption of acidophilus milk (2% fat) containing strains B, N1, and E significantly reduced mean total H2 production compared with that of the control reduced-fat (2% fat) milk, but milk containing strain ATCC 4356 did not differ from the control. Acidophilus milk containing L. acidophilus N1 was the most effective of the four acidophilus milks in improving lactose digestion and tolerance. Strain N1 exhibited the lowest beta-galactosidase activity and lactose transport but the greatest bile and acid tolerance of the four strains. The results indicated that bile and acid tolerance may be important factors to consider when L. acidophilus strains are selected for improving lactose digestion and tolerance.
研究了嗜酸乳杆菌的胆汁敏感性、乳糖转运及耐酸性对乳糖体内消化的影响。使用四株表现出不同程度乳糖转运、β-半乳糖苷酶活性及胆汁敏感性的嗜酸乳杆菌制备未发酵嗜酸乳杆菌奶。通过测量11名乳糖消化不良受试者摄入四种嗜酸乳杆菌测试奶后呼出的氢气量来评估乳糖吸收不良情况。测试餐以随机双盲方案喂食。与对照低脂（2%脂肪）奶相比，饮用含有菌株B、N1和E的嗜酸乳杆菌奶（2%脂肪）显著降低了平均总氢气产生量，但含有菌株ATCC 4356的奶与对照无差异。在四种嗜酸乳杆菌奶中，含有嗜酸乳杆菌N1的奶在改善乳糖消化和耐受性方面最有效。在这四株菌株中，菌株N1表现出最低的β-半乳糖苷酶活性和乳糖转运能力，但具有最强的胆汁和酸耐受性。结果表明，在选择嗜酸乳杆菌菌株以改善乳糖消化和耐受性时，胆汁和酸耐受性可能是需要考虑的重要因素。
