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2
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Biochem J. 1993 Dec 15;296 ( Pt 3)(Pt 3):549-51. doi: 10.1042/bj2960549.
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Cloning and sequence analysis of cDNA for the luminescent protein aequorin.发光蛋白水母发光蛋白cDNA的克隆与序列分析
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Cloning and expression of the cDNA coding for aequorin, a bioluminescent calcium-binding protein.编码水母发光蛋白（一种生物发光钙结合蛋白）的cDNA的克隆与表达。
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Transgenic plant aequorin reports the effects of touch and cold-shock and elicitors on cytoplasmic calcium.转基因植物水母发光蛋白可报告触摸、冷休克和激发子对细胞质钙的影响。
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Peroxidized coelenterazine, the active group in the photoprotein aequorin.过氧化腔肠素，即发光蛋白水母发光蛋白中的活性基团。
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用鱼卵测量腔肠素类似物的膜通透性。

Membrane permeability of coelenterazine analogues measured with fish eggs.

作者信息

Shimomura O

机构信息

Marine Biological Laboratory, Woods Hole, MA 02543, USA.

出版信息

Biochem J. 1997 Sep 1;326 ( Pt 2)(Pt 2):297-8. doi: 10.1042/bj3260297.

DOI:10.1042/bj3260297
PMID:9291095
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC1218668/
Abstract

To determine the suitability of various coelenterazine analogues for the regeneration of aequorin in living cells, the membrane permeabilities of 11 analogues were measured using the eggs of the killifish Fundulus grandis by soaking the eggs in solutions containing the analogues. The results indicated that e-coelenterazine, which has an exceptionally high rate of in vitro regeneration of aequorin, not only permeated poorly into the eggs but also was highly unstable. All other analogues tested permeated sufficiently into the eggs. The highest permeability was found with f-coelenterazine; the concentration of f-coelenterazine in the eggs was about five times that in the surrounding medium, assuming that the distribution of the compound in the egg is uniform.

摘要

为了确定各种腔肠素类似物在活细胞中用于水母发光蛋白再生的适用性，通过将鳉鱼（Fundulus grandis）的卵浸泡在含有类似物的溶液中，测量了11种类似物的膜通透性。结果表明，体外水母发光蛋白再生速率极高的e-腔肠素不仅向卵内的渗透较差，而且非常不稳定。测试的所有其他类似物都能充分渗透到卵内。f-腔肠素的通透性最高；假设该化合物在卵内分布均匀，卵内f-腔肠素的浓度约为周围培养基中的五倍。