Quax W J
Genencor Internationa B.V. Delft, The Netherlands.
Folia Microbiol (Praha). 1997;42(2):99-103. doi: 10.1007/BF02898715.
The heterologous expression of proteins is without doubt one of the most fascinating applications of the recombinant DNA technique. Despite clear successes many attempts to produce a certain protein in a heterologous host cell have met with technical difficulties. Secretion from cells has been used as a solution to overcome the intracellular formation of inactive protein. Microorganisms with a history of use in the fermentation industry exhibit clear advantages over the frequently used Escherichia coli as host cells for secreted products. Interleukin-3, chymosin and phytase are examples of commercial products that are produced efficiently with the aid of industrial microorganisms.
蛋白质的异源表达无疑是重组DNA技术最引人入胜的应用之一。尽管取得了显著成功，但在异源宿主细胞中生产某种蛋白质的许多尝试都遇到了技术难题。细胞分泌已被用作克服无活性蛋白质在细胞内形成的一种解决方案。在发酵工业中有使用历史的微生物作为分泌产物的宿主细胞，比常用的大肠杆菌具有明显优势。白细胞介素-3、凝乳酶和植酸酶是借助工业微生物高效生产的商业产品实例。
