Rizzo M C, Fernández-Caldas E, Solé D, Naspitz C K
Division of Allergy and Immunology, Federal University of São Paulo, Brazil.
J Investig Allergol Clin Immunol. 1997 Jul-Aug;7(4):242-8.
The sensitization to inhaled allergens was studied in 80 Brazilian children and 12 controls, aged 6 to 16 years. We performed skin tests with Alternaria alternata, cat, dog, Lolium perenne, grasses and the following domestic mites: Blomia tropicalis, Dermatophagoides pteronyssinus, Dermatophagoides farinae, Lepidoglyphus destructor, Chortoglyphus arcutus and Aleuroglyphus ovatus. The frequencies of positive skin tests (mean diameter > or = 23 mm) were respectively 15%, 11%, 11%, 6%, 7%, 95%, 92%, 88%, 76%, 75% and 71%. The mean diameter of the wheal to domestic mites was higher to D. pteronyssinus than to B. tropicalis, L. destructor, A. ovatus and C. arcuatus (p < 0.05). The skin test results to domestic mites by regression analysis revealed highly significant correlation (p < 0.0001), suggesting considerable cross-reactivity between them. Specific IgE to each mite was determined and the results expressed as the mean of the percentage of total counts bound (% TCB). We had the highest levels of % TCB to D. farinae, followed by D. pteronyssinus, E. maynei, B. tropicalis, C. arcuatus, L. destructor and A. ovatus. The Brazilian children with asthma had more positive skin tests to domestic mites than to other inhalant allergens, and based on previous studies performed in São Paulo that have shown D. pteronyssinus and B. tropicalis as the most prevalent mite species in house dust, we assumed that IgE Ab to D. farinae reflects cross-reactivity with D. pteronyssinus in most cases. The observed high levels of IgE Ab to domestic mites stress the importance of environmental avoidance measures, decreasing the rate of sensitization or perhaps the development of symptoms of asthma in genetically predisposed children.
对80名6至16岁的巴西儿童和12名对照儿童进行了吸入性变应原致敏情况的研究。我们用链格孢、猫、狗、多年生黑麦草、禾本科植物以及以下家养螨类进行了皮肤试验：热带无爪螨、粉尘螨、屋尘螨、腐食酪螨、嗜卷书虱和椭圆食粉螨。皮肤试验阳性（平均直径≥23毫米）的频率分别为15%、11%、11%、6%、7%、95%、92%、88%、76%、75%和71%。粉尘螨引起的风团平均直径高于热带无爪螨、腐食酪螨、椭圆食粉螨和嗜卷书虱（p<0.05）。通过回归分析得出的家养螨类皮肤试验结果显示出高度显著的相关性（p<0.0001），表明它们之间存在相当程度的交叉反应性。测定了每种螨的特异性IgE，结果以结合的总计数百分比（%TCB）的平均值表示。我们发现屋尘螨的%TCB水平最高，其次是粉尘螨、梅氏嗜霉螨、热带无爪螨、嗜卷书虱、腐食酪螨和椭圆食粉螨。患有哮喘的巴西儿童对家养螨类的皮肤试验阳性率高于其他吸入性变应原，并且基于此前在圣保罗进行的研究表明粉尘螨和热带无爪螨是室内灰尘中最常见的螨种，我们认为在大多数情况下，针对屋尘螨的IgE抗体反映了与粉尘螨的交叉反应性。观察到的针对家养螨类的高IgE抗体水平强调了环境规避措施的重要性，可降低遗传易感性儿童的致敏率或哮喘症状的发生率。
