Söderberg-Nauclér C, Fish K N, Nelson J A
Department of Microbiology and Immunology, Oregon Health Sciences University, Portland 97201-3098, USA.
Cell. 1997 Oct 3;91(1):119-26. doi: 10.1016/s0092-8674(01)80014-3.
Reactivation of human cytomegalovirus (HCMV) results in severe disease in AIDS patients and immunocompromised patients receiving blood transfusions or organ or bone marrow grafts. Although the site of HCMV latency is unknown, blood cells have been implicated as a viral reservoir. In this study, we demonstrate HCMV reactivation in vitro from seven consecutive healthy donors through allogeneic stimulation of peripheral blood mononuclear cells (PBMCs). HCMV replication was detected at 17 days poststimulation, and virus was recovered after long-term culture from a macrophage expressing dendritic cell markers. Thus, these observations demonstrate that PBMCs harbor latent HCMV, which reactivates in a myeloid lineage cell upon allogeneic stimulation.
人巨细胞病毒（HCMV）的再激活会导致艾滋病患者以及接受输血、器官移植或骨髓移植的免疫功能低下患者出现严重疾病。尽管HCMV潜伏的部位尚不清楚，但血细胞被认为是病毒储存库。在本研究中，我们通过对健康供者外周血单个核细胞（PBMC）进行同种异体刺激，在体外证实了来自7名连续健康供者的HCMV再激活。刺激后17天检测到HCMV复制，长期培养后从表达树突状细胞标志物的巨噬细胞中分离出病毒。因此，这些观察结果表明PBMC中存在潜伏的HCMV，其在同种异体刺激后会在髓系细胞中再激活。
