有证据表明，Snf-Swi可调控酿酒酵母中SUC2启动子的TATA和UAS区域的染色质结构。
Evidence that Snf-Swi controls chromatin structure over both the TATA and UAS regions of the SUC2 promoter in Saccharomyces cerevisiae.
作者信息
Wu L, Winston F
机构信息
Department of Genetics, Harvard Medical School, Boston, MA 02115, USA.
出版信息
Nucleic Acids Res. 1997 Nov 1;25(21):4230-4. doi: 10.1093/nar/25.21.4230.
Abstract
The Snf-Swi complex of the yeast Saccharomyces cerevisiae has been shown to control gene expression by controlling chromatin structure. We have analyzed the promoter of the SUC2 gene, a gene strongly controlled by Snf-Swi, by a high resolution analysis of micrococcal nuclease digests. This analysis suggests that there are at least four nucleosomes positioned over the SUC2 TATA and UAS regions under conditions repressing SUC2 transcription. Under derepressing conditions this entire promoter region is much more sensitive to MNase digestion. Analysis of an snf2 Delta mutant demonstrates that even under derepressing conditions the SUC2 promoter is resistant to MNase digestion. Thus, the Snf-Swi complex appears to control chromatin structure over both the SUC2 TATA and UAS regions. The presence of nucleosomes over both promoter regions may explain the strong requirement of SUC2 for Snf-Swi function.
摘要
酿酒酵母的Snf-Swi复合物已被证明可通过控制染色质结构来控制基因表达。我们通过对微球菌核酸酶消化产物进行高分辨率分析，研究了SUC2基因的启动子，SUC2基因受Snf-Swi的强烈调控。该分析表明，在抑制SUC2转录的条件下，至少有四个核小体位于SUC2的TATA和UAS区域上。在去抑制条件下，整个启动子区域对微球菌核酸酶的消化更为敏感。对snf2Δ突变体的分析表明，即使在去抑制条件下，SUC2启动子对微球菌核酸酶的消化也具有抗性。因此，Snf-Swi复合物似乎在SUC2的TATA和UAS区域上控制染色质结构。两个启动子区域上都存在核小体，这可能解释了SUC2对Snf-Swi功能的强烈需求。
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