通过脉冲场电泳对嗜肺军团菌进行鉴别性基因组指纹分析。
Discriminatory genomic fingerprinting of Legionella pneumophila by pulsed-field electrophoresis.
作者信息
Johnson W M, Bernard K, Marrie T J, Tyler S D
机构信息
Laboratory Centre for Disease Control, Health Canada Tunney's Pasture, Ottawa, Ontario.
出版信息
J Clin Microbiol. 1994 Oct;32(10):2620-1. doi: 10.1128/jcm.32.10.2620-2621.1994.
Abstract
Eight strains of Legionella pneumophila were used to optimize cleavage of DNA with BssHII, SalI, or SpeI and separation by pulsed-field electrophoresis. Isolates from a community outbreak involving a contaminated hot tub were genomically identical. Cleavage patterns were distinctly different for unrelated environmental and nosocomial strains from a single hospital.
摘要
使用八株嗜肺军团菌来优化用BssHII、SalI或SpeI进行的DNA切割以及通过脉冲场电泳进行的分离。从一起涉及受污染热水浴缸的社区疫情中分离出的菌株在基因组上是相同的。来自同一家医院的不相关环境菌株和医院感染菌株的切割模式明显不同。
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