Heddle R J, Shearman D J
Clin Exp Immunol. 1979 Oct;38(1):22-30.
It has been proposed that in ulcerative colitis the intestinal flora stimulates autoimmune reactions to colonic epithelium through shared specificities exposed in a `common antigen' found in most . The present experiments aimed to resolve conflicting data as to whether patients with ulcerative colitis have selectively increased serum antibody titres to enterobacterial common antigen or 014, which is rich in enterobacterial common antigen. Antibody titres to enterobacterial common antigen and lipopolysaccharides of 014 and of five serotypes of which occur frequently in human faeces were measured by passive haemagglutination. Sera were obtained from patients with ulcerative colitis, age- and sex-matched controls and subjects with other gastrointestinal disorders. Serum titres to enterobacterial common antigen and 014 lipopolysaccharide were not increased significantly in subjects with ulcerative colitis but significant increases were observed in subjects with chronic liver disease without colitis. Patients with active ulcerative colitis, patients with chronic liver disease and subjects convalescent from or infections all had significantly increased serum titres to the antigens as a group. Class-specific enhancement of passive haemagglutination indicated that the class distribution of serum antibodies was similar in subjects with ulcerative colitis and controls.
有人提出，在溃疡性结肠炎中，肠道菌群通过在大多数“共同抗原”中暴露的共同特异性刺激对结肠上皮的自身免疫反应。目前的实验旨在解决关于溃疡性结肠炎患者血清中针对肠道细菌共同抗原或富含肠道细菌共同抗原的O14的抗体滴度是否选择性升高的相互矛盾的数据。通过被动血凝试验测定了针对肠道细菌共同抗原、O14的脂多糖以及在人类粪便中频繁出现的五种血清型的脂多糖的抗体滴度。血清取自溃疡性结肠炎患者、年龄和性别匹配的对照组以及患有其他胃肠道疾病的受试者。溃疡性结肠炎患者血清中针对肠道细菌共同抗原和O14脂多糖的滴度没有显著升高，但在无结肠炎的慢性肝病患者中观察到显著升高。患有活动性溃疡性结肠炎的患者、慢性肝病患者以及从伤寒或副伤寒感染中康复的受试者作为一个群体，其血清中针对这些抗原的滴度均显著升高。被动血凝试验的类特异性增强表明，溃疡性结肠炎患者和对照组血清抗体的类分布相似。
