流感嗜血杆菌的acrAB同源物编码一种功能性多药外排泵。
The acrAB homolog of Haemophilus influenzae codes for a functional multidrug efflux pump.
作者信息
Sánchez L, Pan W, Viñas M, Nikaido H
机构信息
Department of Molecular and Cell Biology, University of California, Berkeley 94720-3206, USA.
出版信息
J Bacteriol. 1997 Nov;179(21):6855-7. doi: 10.1128/jb.179.21.6855-6857.1997.
Abstract
Disruption of gene HI0894 or HI0895 in Haemophilus influenzae Rd, homologs of Escherichia coli acrAB multidrug efflux genes, caused hypersusceptibility to erythromycin, rifampin, novobiocin, and dyes such as ethidium bromide and crystal violet and increased accumulation of radioactive erythromycin, showing that these genes are expressed and contribute to the baseline level resistance of this organism through active drug efflux. The gene disruption did not produce detectable changes in susceptibility to several other antibiotics, possibly because rapid influx of small antibiotic molecules through the large H. influenzae porin channels counterbalances their efflux.
摘要
流感嗜血杆菌Rd中基因HI0894或HI0895（大肠杆菌acrAB多药外排基因的同源物）的破坏，导致对红霉素、利福平、新生霉素以及诸如溴化乙锭和结晶紫等染料高度敏感，并增加了放射性红霉素的积累，表明这些基因通过主动药物外排发挥作用并有助于该生物体的基线耐药水平。基因破坏对其他几种抗生素的敏感性未产生可检测到的变化，这可能是因为小抗生素分子通过大的流感嗜血杆菌孔蛋白通道的快速流入抵消了它们的外排。
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