Riedel D D, Kaufmann S H
Department of Immunology, Ulm University, Germany.
Infect Immun. 1997 Nov;65(11):4620-3. doi: 10.1128/iai.65.11.4620-4623.1997.
Macrophages (MAC) and polymorphonuclear granulocytes (PNG) are professional phagocytes which perform essential functions in antibacterial defense. The intracellular bacterium Mycobacterium tuberculosis persists and replicates in resting macrophages. Although it is generally assumed that activated MAC are central to protection against M. tuberculosis, PNG may also contribute to defense. We wondered whether PNG produce proinflammatory chemokines after stimulation by M. tuberculosis or its major cell wall component, lipoarabinomannan (LAM). In this study, we showed that M. tuberculosis- and LAM-activated human PNG secrete the leukocyte attractant interleukin-8 (IL-8) and the PNG-specific chemokine GRO-alpha in a dose-dependent manner. Treatment of PNG with the leukotriene-B4 inhibitor MK-886 prior to stimulation with M. tuberculosis or LAM partially blocked IL-8 and GRO-alpha induction, suggesting involvement of the 5-lipoxygenase pathway in the secretion of these chemokines. We conclude that PNG contribute to early resistance to M. tuberculosis via chemokine secretion.
巨噬细胞（MAC）和多形核粒细胞（PNG）是专业吞噬细胞，在抗菌防御中发挥重要作用。细胞内细菌结核分枝杆菌在静息巨噬细胞中持续存在并复制。尽管通常认为活化的MAC对抵抗结核分枝杆菌至关重要，但PNG也可能有助于防御。我们想知道PNG在受到结核分枝杆菌或其主要细胞壁成分脂阿拉伯甘露聚糖（LAM）刺激后是否会产生促炎趋化因子。在本研究中，我们表明结核分枝杆菌和LAM活化的人PNG以剂量依赖方式分泌白细胞吸引剂白细胞介素-8（IL-8）和PNG特异性趋化因子GRO-α。在用结核分枝杆菌或LAM刺激之前，用白三烯-B4抑制剂MK-886处理PNG可部分阻断IL-8和GRO-α的诱导，提示5-脂氧合酶途径参与这些趋化因子的分泌。我们得出结论，PNG通过趋化因子分泌有助于对结核分枝杆菌的早期抵抗。
