Duittoz A H, Batailler M, Caldani M
Faculté des Sciences et Techniques, Université François Rabelais, Tours, France.
J Neuroendocrinol. 1997 Sep;9(9):669-75. doi: 10.1046/j.1365-2826.1997.00627.x.
The aim of this study was to establish an in vitro model of ovine luteinizing hormone-releasing hormone (LHRH) neurones. Olfactory placodes from 26 day-old sheep embryos (E26) were used for explant culture. Cultures were maintained successfully up to 35 days, but were usually used at 17 days for immunocytochemistry. LHRH and neuronal markers such as neurofilament (NF) were detected by immunocytochemistry within and/or outside the explant. Three main types of LHRH positive cells are described: (1) neuroblastic LHRH and NF immunoreactive cells with round cell body and very short neurites found mainly within the explant, (2) migrating LHRH bipolar neurones with an fusiform cell body, found outside the explant, (3) network LHRH neuron, bipolar or multipolar with long neurites connecting other LHRH neurons. Cell morphology was very similar to that which has been described in the adult sheep brain. These results strongly suggest that LHRH neurones in the sheep originate from the olfactory placode. This mode may represent a useful tool to study LHRH neurones directly in the sheep.
本研究的目的是建立绵羊促黄体生成素释放激素（LHRH）神经元的体外模型。使用来自26日龄绵羊胚胎（E26）的嗅基板进行外植体培养。培养物成功维持了35天，但通常在17天时用于免疫细胞化学。通过免疫细胞化学在外植体内和/或外检测到LHRH和神经元标志物，如神经丝（NF）。描述了三种主要类型的LHRH阳性细胞：（1）神经母细胞性LHRH和NF免疫反应性细胞，细胞体呈圆形，神经突非常短，主要在外植体内发现；（2）迁移性LHRH双极神经元，细胞体呈梭形，在外植体外发现；（3）网络LHRH神经元，双极或多极，具有连接其他LHRH神经元的长神经突。细胞形态与成年绵羊脑中描述的非常相似。这些结果强烈表明，绵羊中的LHRH神经元起源于嗅基板。这种模型可能是直接研究绵羊LHRH神经元的有用工具。
