鉴定葡萄球菌γ-溶血素HγII特异性功能所必需的最小片段。

Identification of the minimum segment essential for the H gamma II-specific function of staphylococcal gamma-hemolysin.

作者信息

Nariya H, Kamio Y

机构信息

Department of Applied Biological Chemistry, Faculty of Agriculture, Tohoku University, Sendai, Japan.

出版信息

Biosci Biotechnol Biochem. 1997 Oct;61(10):1786-8. doi: 10.1271/bbb.61.1786.

DOI:10.1271/bbb.61.1786

PMID:9362129

Abstract

Staphylococcal gamma-hemolysin consists of H gamma I (or LukF) of 34 kDa and H gamma II of 32 kDa, which cooperatively lyse human erythrocytes. Our previous data showed that the N-terminal 57-residue segment of H gamma II is the essential region for the H gamma II function [H. Nariya and Y. Kamio, Biosci. Biotech. Biochem., 59, 1603-1604 (1995)]. To identify the minimum amino acid residues in the 57-residue segment responsible for the specific hemolytic activity, a series of mutant genes were constructed and expressed in Escherichia coli. The mutant proteins were purified and assayed for their hemolytic activity. The results indicate that the 5-residue segment (K23R24L25A26I27) of H gamma II is the minimum region essential for the H gamma II function.

摘要

葡萄球菌γ-溶血素由34 kDa的HγI（或LukF）和32 kDa的HγII组成，二者协同作用可溶解人红细胞。我们之前的数据表明，HγII的N端57个氨基酸残基片段是HγII发挥功能的关键区域[H. Nariya和Y. Kamio，《生物科学、生物技术与生物化学》，59，1603 - 1604（1995）]。为了确定这57个氨基酸残基片段中负责特定溶血活性的最小氨基酸残基，构建了一系列突变基因并在大肠杆菌中表达。对突变蛋白进行纯化并检测其溶血活性。结果表明，HγII的5个氨基酸残基片段（K23R24L25A26I27）是HγII发挥功能的最小关键区域。