Giuliano A R, Papenfuss M, Nour M, Canfield L M, Schneider A, Hatch K
Arizona Cancer Center, University of Arizona, Tucson 85724, USA.
Cancer Epidemiol Biomarkers Prev. 1997 Nov;6(11):917-23.
Research from the past several years has definitively shown intermediate and high risk-type human papillomavirus (HPV) infection to play a significant role in cervical carcinogenesis. Persistent compared with intermittent infection appears to confer an elevated risk, and cofactors may be necessary to allow the virus to progress to cervical cancer. We explored the association between circulating concentrations of the antioxidant nutrients (alpha- and beta-carotene, lutein, lycopene, beta-cryptoxanthin, alpha-tocopherol, gamma-tocopherol, and ascorbate) and persistent HPV infection among 123 low-income Hispanic women who were all nonsmokers and were not currently using vitamin and mineral supplements. In addition, the association between these nutrients and grade of cervical pathology, independent of HPV status, was assessed. Intermediate and high risk-type HPV infection was assessed by the Digene Hybrid Capture System at two time points, 3 months apart. At the second interview, cytology, colposcopy, and a fasting blood draw were conducted. Mean concentrations of serum and plasma antioxidant nutrients were calculated within categories of HPV status (two times HPV negative, one time HPV positive, and two times HPV positive) and colposcopy. Adjusted mean concentrations of serum beta-carotene, beta-cryptoxanthin, lutein, and alpha- and gamma-tocopherol were on average 24% (P < 0.05) lower among women two times HPV positive compared with either two times HPV negative or one time HPV positive. Independent of HPV status, alpha-tocopherol was significantly inversely associated with grade of cervical dysplasia (normal, 21.57 microM; cervical intraepithelial neoplasia III, 17.27 microM). The results obtained in this study need to be confirmed in larger cohort studies with a longer follow-up period.
过去几年的研究已明确表明，中高危型人乳头瘤病毒（HPV）感染在宫颈癌发生过程中起重要作用。与间歇性感染相比，持续性感染似乎会增加风险，且可能需要辅助因素才能使病毒发展为宫颈癌。我们在123名低收入西班牙裔女性中进行研究，她们均不吸烟且未服用维生素和矿物质补充剂，探讨抗氧化营养素（α-和β-胡萝卜素、叶黄素、番茄红素、β-隐黄质、α-生育酚、γ-生育酚和抗坏血酸）的循环浓度与持续性HPV感染之间的关联。此外，还评估了这些营养素与宫颈病理分级之间的关联（独立于HPV状态）。通过Digene杂交捕获系统在两个时间点（间隔3个月）评估中高危型HPV感染情况。在第二次访谈时，进行了细胞学检查、阴道镜检查和空腹采血。计算HPV状态类别（两次HPV阴性、一次HPV阳性和两次HPV阳性）和阴道镜检查下血清和血浆抗氧化营养素的平均浓度。与两次HPV阴性或一次HPV阳性的女性相比，两次HPV阳性的女性血清β-胡萝卜素、β-隐黄质、叶黄素以及α-和γ-生育酚的调整后平均浓度平均低24%（P<0.05）。独立于HPV状态，α-生育酚与宫颈发育异常分级呈显著负相关（正常，21.57微摩尔；宫颈上皮内瘤变III级，17.27微摩尔）。本研究获得的结果需要在更大规模、随访期更长的队列研究中得到证实。
