Spedding M, Weetman D F
Br J Pharmacol. 1976 Jun;57(2):305-10. doi: 10.1111/j.1476-5381.1976.tb07480.x.
1 The mechanisms by which adenosine 5'-triphosphate (ATP), adenosine 5'-diphosphate (ADP), adenosine 5'-monophosphate (AMP) and adenosine relax the taenia caecum preparation of the guineapig have been studied. ATP and ADP produced similar effects which were qualitatively different from those of AMP and adenosine. 2 2-2'Pyridylisatogen tosylate (PIT: 50 muM for 30 min) blocked the effects of ATP and ADP, but exhibited weak activity against AMP and failed to antagonize the effects of adenosine. The action of PIT was unaffected by the inclusion of dipyridamole (2muM) in the bathing fluid. 3 There was a significant correlation between the sensitivity of individual preparations to ATP or ADP and the blocking potency of PIT. 4 The presence of adenosine in the bathing fluid (2 mM for greater than 30 min) desensitized the taenia to subsequent applications of adenosine. The effects of ATP were increased by this procedure. 5 The results indicate that ATP and adenosine relax the taenia by different mechanisms.
1 研究了三磷酸腺苷（ATP）、二磷酸腺苷（ADP）、一磷酸腺苷（AMP）和腺苷使豚鼠盲肠带松弛的机制。ATP和ADP产生的效应相似，在性质上与AMP和腺苷的效应不同。2 对甲苯磺酰2-2′-吡啶异染素（PIT：50μM作用30分钟）可阻断ATP和ADP的效应，但对AMP的活性较弱，且不能拮抗腺苷的效应。浴液中加入双嘧达莫（2μM）不影响PIT的作用。3 各制剂对ATP或ADP的敏感性与PIT的阻断效力之间存在显著相关性。4 浴液中存在腺苷（2 mM，作用超过30分钟）会使盲肠带对随后给予的腺苷产生脱敏作用。此操作会增强ATP的效应。5 结果表明，ATP和腺苷通过不同机制使盲肠带松弛。
