Carbone A, Cilia A M, Gloghini A, Canzonieri V, Pastore C, Todesco M, Cozzi M, Perin T, Volpe R, Pinto A, Gaidano G
Division of Pathology, Centro di Riferimento Oncologico, Istituto Nazionale di Ricovero e Cura a Carattere Scientifico, Aviano, Italy.
Int J Cancer. 1997 Nov 14;73(4):562-9. doi: 10.1002/(sici)1097-0215(19971114)73:4<562::aid-ijc18>3.0.co;2-b.
Primary lymphomatous effusions are represented by cases of non-Hodgkin's lymphoma (NHL) which grow in liquid phase in the serous body cavities in the absence of solid tumour masses. Based on morphologic, immunophenotypic, virologic and genotypic features, primary lymphomatous effusions are distinguished into body cavity-based lymphoma (BCBL), Burkitt's lymphoma (BL) and immunoblastic large-cell lymphoma. The histogenesis and pathogenesis of primary lymphomatous effusions are virtually unclarified. In this study, we have established 2 cell lines (CRO-AP/1 and CRO-AP/2) representative of 2 distinct categories of primary lymphomatous effusion, BCBL (CRO-AP/2) and BL (CRO-AP/1). Both CRO-AP/1 and CRO-AP/2 carry monoclonal re-arrangements of the immunoglobulin genes which are identical to those of the respective parental tumours. Consistent with its BCBL origin, CRO-AP/2 is characterised by a non-B, non-T phenotype and harbours infection by HHV-8 (approx. 100 viral copies/cell) and Epstein-Barr virus. Conversely, CRO-AP/1 expresses several B cell-associated antigens, lacks HHV-8 infection and carries the genetic hallmark of BL, i.e., a chromosomal breakpoint of band 8q24. CRO-AP/1 and CRO-AP/2 may be valuable for the biologic characterization of primary lymphomatous effusions, particularly since the number of available cell lines derived from such lymphomatous effusions is extremely limited.
原发性淋巴瘤性积液以非霍奇金淋巴瘤（NHL）病例为代表，这些病例在没有实体肿瘤块的情况下，在浆膜体腔内以液相生长。根据形态学、免疫表型、病毒学和基因特征，原发性淋巴瘤性积液可分为体腔淋巴瘤（BCBL）、伯基特淋巴瘤（BL）和免疫母细胞性大细胞淋巴瘤。原发性淋巴瘤性积液的组织发生和发病机制实际上尚不清楚。在本研究中，我们建立了2种细胞系（CRO-AP/1和CRO-AP/2），分别代表原发性淋巴瘤性积液的2个不同类别，即BCBL（CRO-AP/2）和BL（CRO-AP/1）。CRO-AP/1和CRO-AP/2均携带与各自亲本肿瘤相同的免疫球蛋白基因单克隆重排。与它的BCBL起源一致，CRO-AP/2的特征是具有非B、非T表型，并感染HHV-8（约100个病毒拷贝/细胞）和爱泼斯坦-巴尔病毒。相反，CRO-AP/1表达几种B细胞相关抗原，缺乏HHV-8感染，并具有BL的基因特征，即8q24带的染色体断点。CRO-AP/1和CRO-AP/2对于原发性淋巴瘤性积液的生物学特性研究可能具有重要价值，特别是因为源自此类淋巴瘤性积液的可用细胞系数量极其有限。
