Reshef V, Mizrachi E, Maretzki T, Silberstein C, Loya S, Hizi A, Carmeli S
School of Chemistry, Raymond and Beverly Sackler Faculty of Exact Sciences, Tel Aviv University, Israel.
J Nat Prod. 1997 Dec;60(12):1251-60. doi: 10.1021/np970327m.
Five novel diacylated sulfoglycolipids (1-5) were isolated from the cyanobacterium Scytonema sp. (TAU strain SL-30-1-4) and four novel acylated diglycolipids (6-9) were isolated from the cyanobacterium Oscillatoria raoi (TAU strain IL-76-1-2). These two groups of glycolipids and related known glycolipids isolated from these two and three other strains of cyanobacteria, Phormidium tenue (TAU strain IL-144-1), O. trichoides (TAU strain IL-104-3-2), and O. limnetica (TAU strain NG-4-1-2), were found to inhibit HIV-1 RT enzymatic activity to different extents. The structure elucidation of the various compounds is based on the selective hydrolysis of the glycerol ester moieties, GCMS analysis of the methyl ester derivatives of the liberated fatty acids, homo- and heteronuclear-2D-NMR techniques, and MS. The use of negative-ion FABMS for analyzing the combination and distribution of the fatty acids in glycolipids is demonstrated.
从蓝藻席藻属（TAU菌株SL - 30 - 1 - 4）中分离出5种新型二酰化磺基糖脂（1 - 5），并从蓝藻罗氏颤藻（TAU菌株IL - 76 - 1 - 2）中分离出4种新型酰化二糖脂（6 - 9）。从这两种蓝藻以及另外三种蓝藻，即细胶鞘藻（TAU菌株IL - 144 - 1）、毛颤藻（TAU菌株IL - 104 - 3 - 2）和湖沼颤藻（TAU菌株NG - 4 - 1 - 2）中分离出的这两组糖脂及相关已知糖脂，被发现能不同程度地抑制HIV - 1逆转录酶的酶活性。各种化合物的结构解析基于甘油酯部分的选择性水解、游离脂肪酸甲酯衍生物的气相色谱 - 质谱分析、同核和异核二维核磁共振技术以及质谱分析。展示了使用负离子快原子轰击质谱法分析糖脂中脂肪酸的组合和分布情况。
