Bernert J T, Turner W E, Pirkle J L, Sosnoff C S, Akins J R, Waldrep M K, Ann Q, Covey T R, Whitfield W E, Gunter E W, Miller B B, Patterson D G, Needham L L, Hannon W H, Sampson E J
Division of Environmental Health Laboratory Sciences, National Center for Environmental Health, Centers for Disease Control and Prevention, Atlanta, GA 30333, USA.
Clin Chem. 1997 Dec;43(12):2281-91.
We describe a sensitive and specific method for measuring cotinine in serum by HPLC coupled to an atmospheric pressure chemical ionization tandem mass spectrometer. This method can analyze 100 samples/day on a routine basis, and its limit of detection of 50 ng/L makes it applicable to the analysis of samples from nonsmokers potentially exposed to environmental tobacco smoke. Analytical accuracy has been demonstrated from the analysis of NIST cotinine standards and from comparative analyses by both the current method and gas chromatography/high-resolution mass spectrometry. Precision has been examined through the repetitive analysis of a series of bench and blind QC materials. This method has been applied to the analysis of cotinine in serum samples collected as part of the Third National Health and Nutrition Examination Survey (NHANES III).
我们描述了一种通过高效液相色谱（HPLC）与大气压化学电离串联质谱仪联用测定血清中可替宁的灵敏且特异的方法。该方法常规情况下每天可分析100个样本，其50 ng/L的检测限使其适用于分析可能接触环境烟草烟雾的非吸烟者的样本。通过对美国国家标准与技术研究院（NIST）可替宁标准品的分析以及当前方法与气相色谱/高分辨率质谱法的对比分析，已证明了分析准确性。通过对一系列实验台和盲法质量控制（QC）材料的重复分析检验了精密度。该方法已应用于作为第三次全国健康和营养检查调查（NHANES III）一部分所采集的血清样本中可替宁的分析。
