Darling P, Chan M, Cox A D, Sokol P A
Department of Microbiology and Infectious Diseases, University of Calgary Health Sciences Centre, Alberta, Canada.
Infect Immun. 1998 Feb;66(2):874-7. doi: 10.1128/IAI.66.2.874-877.1998.
Sixty-one Burkholderia cepacia isolates from patients with cystic fibrosis (CF) and four plant isolates were screened for production of the siderophores salicylic acid (SA), pyochelin, cepabactin, and ornibactins and fingerprinted by a PCR-based randomly amplified polymorphic DNA (RAPD) method. Of the 24 RAPD types determined, 22 (92%) were associated with isolates that produced SA, 21 (87%) were associated with isolates that produced ornibactins, 15 (60%) were associated with isolates that produced pyochelin, and 3 (12%) were associated with isolates that produced cepabactin. Of the 24 RAPD types plus 2 phenotypic variants of types 1 and 9, 3 were associated with isolates that produced all four siderophores, 8 were associated with isolates that produced three siderophores, 12 were associated with isolates that produced two siderophores, and 3 were associated with isolates that produced only one siderophore. These results suggest that the numbers and types of siderophores produced by CF isolates of B. cepacia correlate with RAPD type and that SA and ornibactins are the most prevalent siderophores produced.
对61株来自囊性纤维化（CF）患者的洋葱伯克霍尔德菌分离株和4株植物分离株进行筛选，检测其是否产生铁载体水杨酸（SA）、绿脓菌素、洋葱菌素和鸟氨酸菌素，并采用基于聚合酶链反应（PCR）的随机扩增多态性DNA（RAPD）方法进行指纹图谱分析。在确定的24种RAPD类型中，22种（92%）与产生SA的分离株相关，21种（87%）与产生鸟氨酸菌素的分离株相关，15种（60%）与产生绿脓菌素的分离株相关，3种（12%）与产生洋葱菌素的分离株相关。在24种RAPD类型加上类型1和9的2个表型变异株中，3种与产生所有4种铁载体的分离株相关，8种与产生3种铁载体的分离株相关，12种与产生2种铁载体的分离株相关，3种与仅产生1种铁载体的分离株相关。这些结果表明，CF患者的洋葱伯克霍尔德菌分离株产生的铁载体数量和类型与RAPD类型相关，且SA和鸟氨酸菌素是最普遍产生的铁载体。
