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检测肠球菌中万古霉素耐药性的八种方法比较。

Comparison of eight methods to detect vancomycin resistance in enterococci.

作者信息

Endtz H P, Van Den Braak N, Van Belkum A, Goessens W H, Kreft D, Stroebel A B, Verbrugh H A

机构信息

Erasmus University Medical Center Rotterdam, The Netherlands.

出版信息

J Clin Microbiol. 1998 Feb;36(2):592-4. doi: 10.1128/JCM.36.2.592-594.1998.

Abstract

A collection of genetically unrelated vancomycin-resistant enterococci (VRE) including 50 vanA, 15 vanB, 50 vanC1, and 30 vanC2 VRE were used to evaluate the accuracy of eight currently available susceptibility test methods (agar dilution, disk diffusion, E-test, agar screen plate, Vitek GPS-TA and GPS-101, and MicroScan overnight and rapid panels). vanA VRE were detected by all methods. vanB VRE were often not detected by Vitek GPS-TA and MicroScan rapid (sensitivities, 47 and 53%, respectively), though the new Vitek GPS-101 was found to be a significant improvement. E-test and the agar screen were the only two methods detecting all VRE, including the vanC1/C2 VRE.

摘要

收集了一批基因不相关的耐万古霉素肠球菌(VRE),包括50株vanA、15株vanB、50株vanC1和30株vanC2 VRE,用于评估目前可用的八种药敏试验方法(琼脂稀释法、纸片扩散法、E试验、琼脂筛选平板法、Vitek GPS-TA和GPS-101以及MicroScan过夜和快速鉴定板)的准确性。所有方法均检测到vanA VRE。Vitek GPS-TA和MicroScan快速鉴定板常常检测不到vanB VRE(灵敏度分别为47%和53%),不过新的Vitek GPS-101有显著改进。E试验和琼脂筛选平板法是仅有的两种能检测所有VRE的方法,包括vanC1/C2 VRE。

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