De Haas C J, de Vos N M, Visser M R, Snippe H, Verhoef J
Eijkman Winkler Institute for Microbiology, Infectious Diseases, and Inflammation, University Hospital, Utrecht, The Netherlands.
Clin Exp Immunol. 1998 Feb;111(2):286-92. doi: 10.1046/j.1365-2249.1998.00494.x.
To investigate the effects of Mycobacterium tuberculosis on HIV-1 replication, peripheral blood mononuclear cells (PBMC) of bacille Calmette-Guerin (BCG)-vaccinated donors and non-BCG-vaccinated donors were infected in vitro with a lymphotropic isolate of HIV-1 and cultured in the presence of purified protein derivative (PPD). Addition of PPD resulted in enhanced HIV-1 replication and lymphoproliferation in BCG-vaccinated donor PBMC, while PPD had no such effects in control PBMC. HIV-1 replication increased even more when monocytes were removed from PBMC, while lymphoproliferation was decreased. High percentages of monocytes were associated with a decreased HIV-1 replication and proliferation that could not be reversed by addition of antibodies against the cytokines IL-1, transforming growth factor-beta (TGF-beta) or indomethacin. PPD stimulates PBMC to release IL-10, a cytokine known to down-regulate proliferation and HIV-1 replication. PPD-induced effects on proliferation as well as HIV-1 replication could be partially blocked by adding a monoclonal antibody against MHC class II molecules, suggesting that part of the mechanism of PPD-induced enhancement is T memory cell activation.
为研究结核分枝杆菌对HIV-1复制的影响，用一株嗜淋巴细胞性HIV-1分离株体外感染卡介苗（BCG）接种者和未接种BCG者的外周血单个核细胞（PBMC），并在纯化蛋白衍生物（PPD）存在的情况下进行培养。添加PPD导致接种BCG者的PBMC中HIV-1复制增强和淋巴细胞增殖，而PPD对对照PBMC无此作用。当从PBMC中去除单核细胞时，HIV-1复制进一步增加，而淋巴细胞增殖减少。高比例的单核细胞与HIV-1复制和增殖减少相关，添加抗细胞因子IL-1、转化生长因子-β（TGF-β）的抗体或吲哚美辛无法逆转这种情况。PPD刺激PBMC释放IL-10，这是一种已知可下调增殖和HIV-1复制的细胞因子。添加抗MHC II类分子的单克隆抗体可部分阻断PPD对增殖以及HIV-1复制的诱导作用，这表明PPD诱导增强作用的部分机制是T记忆细胞活化。
