Wellings R P, Brockelsby J C, Baker P N
Department of Obstetrics and Gynaecology, Nottingham City Hospital, UK.
J Soc Gynecol Investig. 1998 Jan-Feb;5(1):31-7. doi: 10.1016/s1071-5576(97)00100-7.
To compare the ability of plasma from a population of women with preeclampsia and a population of plasma from women with normal pregnancies to activate four different endothelial cell types.
The secretion of nitrite and 6-keto prostaglandin F1 alpha by four endothelial cell types (isolated from the microvasculature of human decidua and skin, as well as a human umbilical vein endothelial cell line and a bovine coronary microvascular cell line) was assessed following a 24-hour incubation with plasma samples from the two groups.
Nitrite production (an indicator of nitric oxide release) was detectable in only the decidual endothelial cells and the bovine microvascular endothelial cells (B-88), whereas 6-keto prostaglandin F1 alpha (stable metabolite of prostacyclin) was detectable in all cells. Only in the B-88 cells was there a greater production of nitrite or 6-keto prostaglandin F1 alpha in response to incubation with plasma from the preeclamptic patients when compared to plasma from the normotensive controls.
The different responses of various endothelial cell types to the activating effects of plasma from preeclamptic women indicate that another important caveat to be considered when bioassaying for the circulating factor(s) of preeclampsia is the choice of endothelial cell to be studied.
比较子痫前期女性群体的血浆与正常妊娠女性群体的血浆激活四种不同内皮细胞类型的能力。
用两组的血浆样本孵育24小时后，评估四种内皮细胞类型（从人蜕膜和皮肤的微血管分离得到，以及人脐静脉内皮细胞系和牛冠状动脉微血管细胞系）亚硝酸盐和6-酮前列腺素F1α的分泌情况。
仅在蜕膜内皮细胞和牛微血管内皮细胞（B-88）中可检测到亚硝酸盐生成（一氧化氮释放的指标），而在所有细胞中均可检测到6-酮前列腺素F1α（前列环素的稳定代谢产物）。与血压正常对照组的血浆相比，仅在B-88细胞中，与子痫前期患者的血浆孵育后，亚硝酸盐或6-酮前列腺素F1α的生成量更多。
各种内皮细胞类型对来自子痫前期女性血浆的激活作用有不同反应，这表明在检测子痫前期循环因子时，另一个需要考虑的重要注意事项是所研究内皮细胞的选择。
