Nicholson W L, Muir S, Sumner J W, Childs J E
Division of Viral and Rickettsial Diseases, National Center for Infectious Diseases, Centers for Disease Control and Prevention, Atlanta, Georgia 30333, USA.
J Clin Microbiol. 1998 Mar;36(3):695-700. doi: 10.1128/JCM.36.3.695-700.1998.
Rodent (Muridae: Sigmodontinae) blood and sera collected from 14 states were tested for seroreactivity to a cultured isolate of the human granulocytic ehrlichiosis (HGE) agent by using an indirect immunofluorescence assay. Of the 1,240 samples tested, 136 (11%) were found to be reactive at titers of > or = 32. Rodents with HGE agent-specific antibodies were found in New York (23% of 491 samples; geometric mean endpoint titer [GMT] = 441), Connecticut (11% of 100 samples; GMT = 481), California (9% of 32 samples; GMT = 323), Colorado (2% of 212 samples; GMT = 256), Florida (7% of 27 samples; GMT = 362), Maryland (7% of 15 samples; titer = 64), New Jersey (4% of 76 samples; titer = 256), and Wisconsin (13% of 8 samples; titer = 128). Samples from Georgia (n = 16), Illinois (n = 27), Nevada (n = 27), North Carolina (n = 52), Ohio (n = 57), and Utah (n = 100) were not reactive. The earliest seroreactive sample was from a Peromyscus leucopus mouse collected in June 1986 in Connecticut, and the majority of the seroreactive samples (68%) were from this species. Samples from other Peromyscus species (P. boylii, P. maniculatus, and P. gossypinus) were also found to be reactive, with a GMT for the genus of 410. Several species of Neotoma woodrats (N. fuscipes, N. lepida, N. albigula, and N. mexicana) from California and Colorado had antibodies that reacted with the HGE agent (genus GMT = 194), suggesting that enzootic cycles of Ehrlichia spp. exist outside of the areas of confirmed human disease. Attempts to amplify and detect ehrlichial DNA from the limited tissues available (n = 40 animals) were unsuccessful. Further studies are needed to determine the identity of the organisms inducing antibody production in these rodent species and to elucidate the epidemiology and public health importance of these agents.
采用间接免疫荧光试验，对从14个州采集的啮齿动物（鼠科：稻鼠亚科）血液和血清进行检测，以确定其对人类粒细胞埃立克体病（HGE）病原体培养分离株的血清反应性。在检测的1240份样本中，有136份（11%）在滴度≥32时呈反应性。在纽约（491份样本中的23%；几何平均终点滴度[GMT]=441）、康涅狄格州（100份样本中的11%；GMT=481）、加利福尼亚州（32份样本中的9%；GMT=323）、科罗拉多州（212份样本中的2%；GMT=256）、佛罗里达州（27份样本中的7%；GMT=362）、马里兰州（15份样本中的7%；滴度=64）、新泽西州（76份样本中的4%；滴度=256）和威斯康星州（8份样本中的13%；滴度=128）发现带有HGE病原体特异性抗体的啮齿动物。来自佐治亚州（n=16）、伊利诺伊州（n=27）、内华达州（n=27）、北卡罗来纳州（n=52）、俄亥俄州（n=57）和犹他州（n=100）的样本无反应性。最早的血清反应性样本来自1986年6月在康涅狄格州捕获的一只白足鼠，大多数血清反应性样本（68%）也来自该物种。来自其他白足鼠物种（博氏白足鼠、北美鹿鼠和棉鼠）的样本也呈反应性，该属的GMT为410。来自加利福尼亚州和科罗拉多州的几种林鼠（棕林鼠、淡褐林鼠、白喉林鼠和墨西哥林鼠）带有与HGE病原体反应的抗体（属GMT=
