通过聚合酶链反应（PCR）检测艾滋病患者肛周溃疡中的单纯疱疹病毒并进行直接分型
Detection and direct typing of herpes simplex virus in perianal ulcers of patients with AIDS by PCR.
作者信息
do Nascimento M C, Sumita L M, de Souza V A, Pannuti C S
机构信息
Instituto de Infectologia Emílio Ribas, São Paulo, SP, Brazil.
出版信息
J Clin Microbiol. 1998 Mar;36(3):848-9. doi: 10.1128/JCM.36.3.848-849.1998.
Abstract
The presence of herpes simplex virus type 1 (HSV-1) and HSV-2 in perianal ulcerations of 41 AIDS patients was assessed by virus culture and a type-specific PCR-based assay. HSV was isolated from the lesion site in 24 of 41 (58.5%) patients, and HSV DNA was detected by PCR in all 24 (100%) of these specimens. Additionally, PCR was used to detect HSV DNA in 12 of 17 (70.5%) HSV culture-negative samples. Thus, HSV genomic sequences could be demonstrated in 36 of 41 (87.8%) perianal ulcers in this series. Full agreement in HSV typing by either immunodot assay or PCR was seen in 24 samples that were positive by both virus culture and PCR. HSV-2 was demonstrated in 35 of 36 (97.2%) HSV-positive samples.
摘要
通过病毒培养和基于型特异性聚合酶链反应（PCR）的检测方法，对41例艾滋病患者肛周溃疡处单纯疱疹病毒1型（HSV-1）和单纯疱疹病毒2型（HSV-2）的存在情况进行了评估。41例患者中有24例（58.5%）从病变部位分离出HSV，在所有这24份标本中（100%）通过PCR检测到HSV DNA。此外，在17份HSV培养阴性样本中的12份（70.5%）中，使用PCR检测HSV DNA。因此，在该系列的41例肛周溃疡中，有36例（87.8%）可检测到HSV基因组序列。在病毒培养和PCR均呈阳性的24份样本中，通过免疫斑点试验或PCR进行的HSV分型完全一致。在36份HSV阳性样本中的35份（97.2%）检测到HSV-2。
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